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4-硝基苯基磷酸盐二钠盐六水合物,4-Nitrophenyl phosphate disodium salt hexahydrate [pNPP二钠六水合物];suitable for cell culture, ≥97%

别名:pNPP二钠六水合物;4-硝基苯磷酸二钠六水合物;对硝基苯磷酸二钠;对硝基苯磷酸盐二钠六水合物;4-Nitrophenyl phosphate disodium salt hexahydrate;p-NPP

CAS号 333338-18-4 线性分子式 O2NC6H4OP(O)(ONa)2 · 6H2O 分子量 371.14 Beilstein/REAXYS Number 5324661 EINECS 编号 224-246-5 MDL number MFCD00066288 PubChem Substance ID 24897708 NACRES NA.75
货号(SKU): N4645
CAS号: 333338-18-4
MDL号: MFCD00066288
增值税发票 √ 免费货运 [顺丰 联邦] √
¥868.94

产品描述

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
• as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)[2]
• to treat adherent cells in adhesion assay[3]
• as a phosphatase substrate in in vitro phosphatase assay[5] in substrate buffer for ELISA[4]

用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应,并在 405nm 下读数。

包装

1, 5 g in poly bottle

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.[1]

性质

Quality Level200
biological sourcesynthetic (organic)
product lineBioReagent
assay≥97%
formpowder
application(s)cell culture | mammalian: suitable
solubilityH2O: 100 mg/mL
storage temp.−20°C
SMILES stringO.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI keyKAKKHKRHCKCAGH-UHFFFAOYSA-L


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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