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葡聚糖凝胶,Sephadex G-10 [G10, GE Healthcare Cytiva]

别名:葡聚糖凝胶G-10;葡聚糖凝胶G10;(安)法玛西亚;Amersharm Pharmacia Biotech;17001001;17001002;17001003;17-0010-01;17-0010-02;17-0010-03

CAS号 9050-68-4 MDL number MFCD00132768 NACRES NA.56
货号(SKU): 17001001
CAS号: 9050-68-4
MDL号: MFCD00132768
增值税发票 √ 免费货运 [顺丰 联邦] √
$229.92

产品描述

葡聚糖凝胶,即Sephadex,是一种凝胶过滤树脂,一种具有三维网状结构的高分子化合物,由葡聚糖加入交联剂通过醚键互相交联聚合而成。不同类型的Sephadex的交联度不同,因此其溶胀度和分子分级范围也不同。交联度越大,网孔结构就越紧密,吸水后的体积膨胀就越小,能允许进入凝胶内部的分子的分子量也就越小,反之亦然。层析时,大于凝胶孔径的大分子,被阻于凝胶相外,沿着凝胶颗粒之间的间隙流动,下移速度最快,故最先被洗脱下来;中分子物质进入凝胶内部,洗脱速度为其次;而小分子物质因全部进入凝胶,所受阻力最大,故最后被洗脱下来,藉此原理物质得到分离。

General description

Sephadex G-10 is a well established gel filtration resin for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight. Smallest exclusion limit in the Sephadex range.

Application

Sephadex ® G-10 是凝胶过滤色谱和蛋白质色谱中使用的凝胶过滤介质。葡聚糖凝胶 ® G-10 已被用于癌症研究,并测定自来水、河水和绿茶样品中的氟化物含量。

性质

Sephadex G-10 is a well established gel filtration resin for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight. Smallest exclusion limit in the Sephadex range.

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 700 molecular weight
  • Efficient removal of contaminants such as salts, dyes, and radioactive labels
  • Lowest exclusion limit (700 molecular weight) in the Sephadex G-range

Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, resin, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency fractionation with shorter diffusion distances. Coarse and resin are are preferred for large scale group separations where high flow rates and low operating pressures are required.

ParameterSephadex G-10
MatrixCross-linked dextran 
Particle size distribution, wet55–165 µm 
Particle Size Dry40 µm-120 µm 
pH stability, operational12–13 
Flow rate2< Darcy´s Law 
Pressure/Flow Specification24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d 
Fractionation range (Mr) (globular proteins)<7 × 10² 
Fractionation (Mp) Dextrans<< 7 x 10² 
Exclusion Limit> 700 
Storage4 to 30°C 
AutoclavableIn wet form (pH 7.0) at 120°C for 30 min
  • 1pH range where resin can be operated without significant change in function.
  • 2The beads behave as rigid spheres obeying Darcy's Law.


安全信息

RIDADR
NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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