StrepTactin™ Sepharose High Performance resin is designed for Strep-tag II™ recombinant protein purification.
产生高纯度的标记重组蛋白,以浓缩的小体积洗脱。生理条件和温和的洗脱保留了目标蛋白的活性。使用 0.5 M NaOH 快速轻松地再生。与多种还原剂、洗涤剂、变性剂和其他添加剂相容。在所有常用的缓冲液中稳定。StrepTactin Sepharose 琼脂糖树脂Strep-tag II 很小 (8 aa),这通常使得纯化后不需要去除标签。 StrepTactin Sepharose High Performance 专为对 Strep-tag II 具有高特异性而开发,以确保纯度。
树脂的小珠粒 (34 μm) 允许以窄峰洗脱。纯化和洗脱条件设计为温和且模拟生理条件,保留目标蛋白的活性。
这种坚固且稳定的 Sepharose 基树脂可实现可重现的纯化,并将进一步浓缩步骤的需要降至最低。请参阅我们的 StrepTactin Sepharose 应用说明,了解纯度超过 95% 的单步纯化示例。
在已发表的研究中成功使用StrepTactin Sepharose 可用于多种应用中的标记蛋白纯化,包括:
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Strep-tag II is small (8 aa), which often makes tag removal unnecessary after purification. StrepTactin Sepharose High Performance has been specially developed for high specificity towards Strep-tag II to ensure purity.
The resin’s small bead size (34 μm) allows elution in narrow peaks. Purification and elution conditions are designed to be mild and mimic physiological conditions, preserving the target protein’s activity.
This robust and stable Sepharose-based resin yields reproducible purifications and minimizes the need for further concentration steps. See our StrepTactin Sepharose application note for an example of a single-step purification that achieved greater than 95% purity.
StrepTactin Sepharose is used for tagged protein purification in a variety of applications, including:
Cytiva solutions include a variety of empty columns for packing your own columns. StrepTactin Sepharose is compatible for use with Tricorn and XK columns.
StrepTactin Sepharose High Performance is also available in a prepacked format: