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RPMI-1640培养基,RPMI-1640 Medium;Hybri-Max™, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without sodium bicarbonate, powder, suitable for hybridoma

Hybri-Max™,改性,含L-谷氨酰胺、4500 mg/L葡萄糖和15mM HEPES,不含碳酸氢钠,粉末型,适用于杂交瘤;别名:Roswell Park Memorial Institute 1640 medium; MDL编号: MFCD00217820 NACRES: NA.75
货号(SKU): R8005
MDL号: MFCD00217820
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¥1,572.96

说明

RPMI 1640培养基由Moore和他的同事于1966年在罗斯威尔公园纪念研究所开发。 作为McCoy 5A培养基的改良版,它被配制用于支持悬浮培养中的淋巴母细胞,但后来被证明支持多种依赖贴壁的细胞。 最初打算与血清补充剂一起使用,RPMI 1640已被证明在没有血清的情况下支持多种细胞系。 它还广泛用于融合方案和杂交细胞的生长。 该培养基适用于培养人类正常和肿瘤性白细胞。

应用

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
 

数量

按照每升培养基16.4克粉末配制。
 

重悬

补充2.0 g/L碳酸氢钠。
 

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
 
 

属性

质量水平

500

形式

powder

technique(s)

cell culture | hybridoma: suitable

杂质

endotoxin

组分

NaHCO3: no
glucose: 4.5 g/L (Dextro)
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
phenol red: 0.0053 g/L

运输

ambient

储存温度

2-8°C

 

安全信息

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

监管信息

动植物来源培养基
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16
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