MacroCap Q is a anion exchanger designed for the purification of large biomolecules such as polyethylene glycol (PEG)-modified proteins (i.e., PEGylated proteins) that are intended for use as biopharmaceuticals.
用于纯化大生物分子,例如用作生物药物的聚乙二醇 (PEG) 修饰的蛋白质(聚乙二醇化蛋白质)。高度多孔的基质提供出色的样品传质和大表面积,以最大限度地吸附生物分子。允许在高负载条件下以高选择性分离单、寡聚和非聚乙二醇化蛋白质。良好的原位清洗 (CIP) 稳定性,可实现较长的树脂寿命和出色的工艺经济性。在高样品负载下获得高纯度和高产率的聚乙二醇化蛋白质。良好的稳定性允许从实验室到生产的可扩展使用。基础基质是高度多孔的,这为吸附大分子提供了高可用表面积。单聚乙二醇化蛋白质可以在单次运行中以高选择性和高上样量从寡聚和非聚乙二醇化蛋白质中分离出高纯度。大孔径也使 MacroCap Q 适用于结合其他大蛋白。
MacroCap 出色的化学稳定性允许在酸性和碱性条件下进行原位清洁。基础基质的亲水性降低了非特异性结合,并减少了疏水性更强的基础基质可能遇到的污染问题。这些特点共同确保了树脂的长寿命。
The base matrix is highly porous, which gives high available surface area for adsorption of large molecules. Mono-PEGylated proteins can be separated to high purity from oligo- and non-PEGylated proteins in with high selectivity and high sample load in a single run. The large pore size also makes MacroCap Q suitable for binding other large proteins.
The excellent chemical stability of MacroCap allows cleaning-in-place to be performed both in acidic and alkaline conditions. The hydrophilic nature of the base matrix reduces nonspecific binding and reduces fouling issues that may be experienced with more hydrophobic base matrices. Together, these features assure long resin lifetime.