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阴离子交换树脂,MacroCap Q

MacroCap Q是一种阴离子交换剂,设计用于纯化大生物分子,例如用作生物药物的聚乙二醇 (PEG) 修饰的蛋白质(即聚乙二醇化蛋白质)。17546904;17546902;17546901
货号(SKU): 17546902
增值税发票 √ 免费送货 √ 订货电话:010-68689484
₹ 17,347.21

MacroCap Q is a anion exchanger designed for the purification of large biomolecules such as polyethylene glycol (PEG)-modified proteins (i.e., PEGylated proteins) that are intended for use as biopharmaceuticals.

用于纯化大生物分子,例如用作生物药物的聚乙二醇 (PEG) 修饰的蛋白质(聚乙二醇化蛋白质)。
高度多孔的基质提供出色的样品传质和大表面积,以最大限度地吸附生物分子。
允许在高负载条件下以高选择性分离单、寡聚和非聚乙二醇化蛋白质。
良好的原位清洗 (CIP) 稳定性,可实现较长的树脂寿命和出色的工艺经济性。
在高样品负载下获得高纯度和高产率的聚乙二醇化蛋白质。
良好的稳定性允许从实验室到生产的可扩展使用。
基础基质是高度多孔的,这为吸附大分子提供了高可用表面积。单聚乙二醇化蛋白质可以在单次运行中以高选择性和高上样量从寡聚和非聚乙二醇化蛋白质中分离出高纯度。大孔径也使 MacroCap Q 适用于结合其他大蛋白。

MacroCap 出色的化学稳定性允许在酸性和碱性条件下进行原位清洁。基础基质的亲水性降低了非特异性结合,并减少了疏水性更强的基础基质可能遇到的污染问题。这些特点共同确保了树脂的长寿命。

 

  • For purification of large biomolecules such as polyethylene glycol (PEG)-modified proteins (PEGylated proteins) intended for use as biopharmaceuticals.
  • Highly porous matrix provides excellent mass transfer of sample and large surface area for maximized adsorption of biomolecules.
  • Allows separation of mono- from oligo- and non-PEGylated proteins with high selectivity under high load conditions.
  • Good cleaning-in-place (CIP) stability for long resin lifetime and excellent process economy.
  • High purity and yield of PEGylated proteins at high sample loads.
  • Good stability allowing for scalable use from laboratory to production.

The base matrix is highly porous, which gives high available surface area for adsorption of large molecules. Mono-PEGylated proteins can be separated to high purity from oligo- and non-PEGylated proteins in with high selectivity and high sample load in a single run. The large pore size also makes MacroCap Q suitable for binding other large proteins.

The excellent chemical stability of MacroCap allows cleaning-in-place to be performed both in acidic and alkaline conditions. The hydrophilic nature of the base matrix reduces nonspecific binding and reduces fouling issues that may be experienced with more hydrophobic base matrices. Together, these features assure long resin lifetime.

 

ParameterMacroCap Q, 500 mL
Chromatography techniqueIon Exchange Chromatography 
BioProcess resinYes 
Particle size, d50V1~50 µm 
Ion Exchanger TypeStrong anion exchanger 
MatrixCross-linked copolymer of allyl dextran and N,N-methylene bisacrylamide 
Ionic Capacity0.07-0.10 mmol H+/ml medium 
pH stability, operational23–10 
pH stability, CIP31–12 
Flow rate< 120 cm/h in BPG 300, 20-cm bed height or 70 cm/h in BPG 300, 30-cm bed height using process buffers with the same viscosity as water at v 3 bar (0.3 MPa) 
Storage4 to 30°C in 20% Ethanol 
Chemical stabilityCommonly used aqueous buffers, 0.5 M NaOH, 0.1 M citric acid, 25% ethanol, 30% propanol, 30% methanol, 50% ethylene glycol, 1% Tween-20, and 1% SDS 
Charged Groups-N(CH3)3+ 
Pack size500 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16