Calmodulin Sepharose 4B affinity resin for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.
用于天然钙调素结合蛋白的一步纯化适用于蛋白质复合物的串联亲和纯化 (TAP)从所有真核细胞中纯化钙调蛋白调节蛋白钙调蛋白是一种高度保守的调节蛋白，存在于所有真核细胞中。该蛋白参与许多细胞过程，如糖原代谢、细胞骨架控制、神经传递、磷酸盐活性和 NAD₊/NADP₊ 的控制。钙调蛋白主要通过蛋白质与疏水性相互作用来结合蛋白质 其表面上的位置。 这些位点在由 Ca 2 ₊ 在单独的 Ca 2 ₊ 结合位点上的作用引起的构象变化后暴露出来。 如果存在酶底物并且酶-底物钙模蛋白-Ca2₊ 复合物特别稳定，则酶的结合可能会增强
Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD₊/NADP₊ Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca₂₊ on separate Ca₂₊-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca₂₊ complexes are particularly stable