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钙调蛋白琼脂糖凝胶4B,Calmodulin Sepharose 4B

Calmodulin Sepharose 4B 亲和树脂,用于纯化对钙调蛋白具有亲和性的蛋白质,钙调蛋白是一种高度保守的调节蛋白,参与真核细胞的许多细胞过程。17052901
货号(SKU): 17052901
增值税发票 √ 免费送货 √ 订货电话:010-68689484
₹ 60,631.47

Calmodulin Sepharose 4B affinity resin for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.

用于天然钙调素结合蛋白的一步纯化
适用于蛋白质复合物的串联亲和纯化 (TAP)
从所有真核细胞中纯化钙调蛋白调节蛋白
钙调蛋白是一种高度保守的调节蛋白,存在于所有真核细胞中。该蛋白参与许多细胞过程,如糖原代谢、细胞骨架控制、神经传递、磷酸盐活性和 NAD₊/NADP₊ 的控制。钙调蛋白主要通过蛋白质与疏水性相互作用来结合蛋白质 其表面上的位置。 这些位点在由 Ca 2 ₊ 在单独的 Ca 2 ₊ 结合位点上的作用引起的构象变化后暴露出来。 如果存在酶底物并且酶-底物钙模蛋白-Ca2₊ 复合物特别稳定,则酶的结合可能会增强

  • For single-step purification of native calmodulin-binding proteins
  • Suitable for tandem affinity purification (TAP) of protein complexes
  • Purification of calmodulin-regulated proteins from all eukaryotic cells

Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD₊/NADP₊ Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca₂₊ on separate Ca₂₊-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca₂₊ complexes are particularly stable

 

ParameterCalmodulin Sepharose 4B, 10 mL
Chromatography techniqueGroup specific affinity 
LigandCalmodulin 
Particle size, d50V1~90 µm 
MatrixAgarose, 4% 
Particle Size45 μm-165 μm 
Ligand Coupling MethodCyanogen bromide activation 
pH stability, operational24–9 
pH stability, CIP34–9 
Flow velocity< 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityStable to commonly used aqueous solutions 
Pack size10 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32