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钙调蛋白琼脂糖凝胶4B，Calmodulin Sepharose 4B

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Calmodulin Sepharose 4B 亲和树脂，用于纯化对钙调蛋白具有亲和性的蛋白质，钙调蛋白是一种高度保守的调节蛋白，参与真核细胞的许多细胞过程。17052901

制造商品牌: (安)法玛西亚 Amersham Pharmacia , GE 生命科学 [思拓凡 Cytiva]

货号（SKU）: 17052901

增值税发票 √ 免费送货 √ 订货电话：010-68689484

目录价.SKU-Variant

10mL

$1,089.89

数量:

Calmodulin Sepharose 4B 钙调蛋白琼脂糖凝胶4B

Calmodulin Sepharose 4B affinity resin for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.

用于天然钙调素结合蛋白的一步纯化
适用于蛋白质复合物的串联亲和纯化 (TAP)
从所有真核细胞中纯化钙调蛋白调节蛋白
钙调蛋白是一种高度保守的调节蛋白，存在于所有真核细胞中。该蛋白参与许多细胞过程，如糖原代谢、细胞骨架控制、神经传递、磷酸盐活性和 NAD₊/NADP₊ 的控制。钙调蛋白主要通过蛋白质与疏水性相互作用来结合蛋白质其表面上的位置。这些位点在由 Ca 2 ₊ 在单独的 Ca 2 ₊ 结合位点上的作用引起的构象变化后暴露出来。如果存在酶底物并且酶-底物钙模蛋白-Ca2₊ 复合物特别稳定，则酶的结合可能会增强

For single-step purification of native calmodulin-binding proteins
Suitable for tandem affinity purification (TAP) of protein complexes
Purification of calmodulin-regulated proteins from all eukaryotic cells

Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD₊/NADP₊ Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca₂₊ on separate Ca₂₊-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca₂₊ complexes are particularly stable

Parameter	Calmodulin Sepharose 4B, 10 mL
Chromatography technique	Group specific affinity
Ligand	Calmodulin
Particle size, d_50V¹	~90 µm
Matrix	Agarose, 4%
Particle Size	45 μm-165 μm
Ligand Coupling Method	Cyanogen bromide activation
pH stability, operational²	4–9
pH stability, CIP³	4–9
Flow velocity	< 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
Storage	2 to 8°C, 20% Ethanol
Chemical stability	Stable to commonly used aqueous solutions
Pack size	10 mL

¹Median particle size of the cumulative volume distribution.
²pH range where resin can be operated without significant change in function.
³pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.

商品规格

属性名称	属性值
储存温度 Storage temp.	2-8°C冷藏
全球实时库存 Availability √	美国St. Louis ≥ 99 \| 欧洲Eur. ≥ 10 \| 東京Tokyo ≥ 22 \| 香港与北京 ≥ 32

钙调蛋白琼脂糖凝胶4B，Calmodulin Sepharose 4B

Calmodulin Sepharose 4B 钙调蛋白琼脂糖凝胶4B

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