Sephadex ® G-100 适用于离心柱纯化 DNA（来自污染的小分子），用于杂交。采用凝胶过滤法分离纯化香蕉中多酚氧化酶。
“Sephadex ® G-100，中型”是一种经常被引用的凝胶过滤介质，用于非常大分子的脱盐和缓冲液交换。Sephadex® 是由葡聚糖与环氧氯丙烷交联而成。Sephadex的交联程度不同，因此其溶胀程度和分子分馏范围也不同。“中型”表示在需要高流速和低操作压力的大规模群分离中使用的大粒径。
Features and Benefits
Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.
- For DNA purification from small molecules by gel filtration in spin columns with an exclusion limit of 25bp ds DNA
- Tested to ensure reproducibly high recovery of DNA
- Suitable for researchers who prefer to prepare their own columns for nucleic acid purification
- Sephadex G-50 DNA Grade is used in AutoSeq G-50 columns, ProbeQuant G-50 Micro Columns, MicroSpin G-50 Columns, and NICK Columns
Sephadex G-100 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Spin columns packed with Sephadex G-100 DNA Grade allow for high-recovery nucleic acid purification via gel filtration. Gel filtration resins do not exhibit a fixed exclusion limit when used in a spin-column format. In spin-column chromatography, the observed exclusion properties that allow the product to pass through the gel while the smaller impurities are retained depend on experimental factors, such as the resin used, sample volume, product size, and the g-forces used in the purification process.
Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.
All these Sephadex types are therefore highly suitable for the purification of oligonucleotides or small DNA fragments following synthesis or in labeling applications.
Sephadex G-50 DNA Grade, and Sephadex G-25 are also sold separately.
|Parameter||illustra Sephadex G-100 DNA Grade SF|
|Particle Size Dry||20 µm-50 µm |
|Pressure/Flow Specification||0.096 bar |
|Fractionation range (Mr) (globular proteins)||4000–100000 |
|Fractionation (Mp) Dextrans||1000-100000 |
|Certificate of Analysis||Yes |
|Pack size||100 g |