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葡聚糖凝胶,Sephadex G-100 DNA Grade [G100, DNA级 GE]

别名:葡聚糖凝胶G-100;葡聚糖凝胶G100;(安)法玛西亚;Amersharm Pharmacia Biotech;17057402;17-0574-02

CAS号 9050-94-6 MDL number MFCD00132235
货号(SKU): 17057402
CAS号: 9050-94-6
MDL号: MFCD00132235
增值税发票 √ 免费货运 [顺丰 联邦] √
$343.63

产品描述

葡聚糖凝胶,即Sephadex,是一种凝胶过滤树脂,一种具有三维网状结构的高分子化合物,由葡聚糖加入交联剂通过醚键互相交联聚合而成。不同类型的Sephadex的交联度不同,因此其溶胀度和分子分级范围也不同。交联度越大,网孔结构就越紧密,吸水后的体积膨胀就越小,能允许进入凝胶内部的分子的分子量也就越小,反之亦然。层析时,大于凝胶孔径的大分子,被阻于凝胶相外,沿着凝胶颗粒之间的间隙流动,下移速度最快,故最先被洗脱下来;中分子物质进入凝胶内部,洗脱速度为其次;而小分子物质因全部进入凝胶,所受阻力最大,故最后被洗脱下来,藉此原理物质得到分离。

General description

Sephadex ® G-100 适用于离心柱纯化 DNA(来自污染的小分子),用于杂交。采用凝胶过滤法分离纯化香蕉中多酚氧化酶。

Application

“Sephadex ® G-100,中型”是一种经常被引用的凝胶过滤介质,用于非常大分子的脱盐和缓冲液交换。Sephadex® 是由葡聚糖与环氧氯丙烷交联而成。Sephadex的交联程度不同,因此其溶胀程度和分子分馏范围也不同。“中型”表示在需要高流速和低操作压力的大规模群分离中使用的大粒径。

Features and Benefits

• 极佳的回收率
• 最小样品稀释度
• 快速且易于使用

性质

Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.

  • For DNA purification from small molecules by gel filtration in spin columns with an exclusion limit of 25bp ds DNA
  • Tested to ensure reproducibly high recovery of DNA
  • Suitable for researchers who prefer to prepare their own columns for nucleic acid purification
  • Sephadex G-50 DNA Grade is used in AutoSeq G-50 columns, ProbeQuant G-50 Micro Columns, MicroSpin G-50 Columns, and NICK Columns

Sephadex G-100 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Spin columns packed with Sephadex G-100 DNA Grade allow for high-recovery nucleic acid purification via gel filtration. Gel filtration resins do not exhibit a fixed exclusion limit when used in a spin-column format. In spin-column chromatography, the observed exclusion properties that allow the product to pass through the gel while the smaller impurities are retained depend on experimental factors, such as the resin used, sample volume, product size, and the g-forces used in the purification process.

Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.

All these Sephadex types are therefore highly suitable for the purification of oligonucleotides or small DNA fragments following synthesis or in labeling applications.

Sephadex G-50 DNA Grade, and Sephadex G-25 are also sold separately.

Parameterillustra Sephadex G-100 DNA Grade SF
Particle Size Dry20 µm-50 µm 
Pressure/Flow Specification0.096 bar 
Fractionation range (Mr) (globular proteins)4000–100000 
Fractionation (Mp) Dextrans1000-100000 
Certificate of AnalysisYes 
Pack size100 g 


安全信息

RIDADR
NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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