脂肪酶来源于米曲霉，Lipase from Aspergillus oryzae；lyophilized, powder, white, ~50 U/mg

Lipase is a carboxylesterase, which belongs to the α/β hydrolase fold family. It consists of a lid domain, hinge domain, and a catalytic triad Ser-His-Asp/Glu. Aspergillus oryzae lipase has a three-dimensional structure is an α/β hydrolase fold similar to that of the esterase enzyme.[1]

 

Lipase from Aspergillus oryzae has been used as a component of mixed enzyme solution to determine the viable cell numbers in the biofilms formed by Listeria monocytogenes strains.[2]

 

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.[3]

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.[4] Aspergillus oryzae lipase might be used in the production of biodiesel from waste cooking oil.[5] It is used in the acetylation of cellulose and ochratoxin A degradation, and asymmetric organic synthesis.[1] Lipase from Aspergillus oryzae is an industrial enzyme that has various uses in food, detergent, and pharmaceutical industries.[5][1] It is useful in immobilization studies for 1,3-dioleoyl-2-palmitoylglycerol (OPO) synthesis.[6]

 

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Cat. No. 62314 as substrate)