General description
Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.[4]
Application
Spermidine has been used in the preparation of gold beads[6] and as a component in nuclei isolation buffer (NIB).[7]
精胺适合用于分子生物缓冲液和> 100 bp DNA沉淀。它被用来在植物和哺乳动物细胞中,通过粒子轰击技术,传递DNA。[1][2]Biochem/physiol Actions
Spermidine helps to maintain the proper activity of gene expression.[4] This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300).[3] Spermidine participates in cell growth.[5]
抑制神经元型一氧化氮合成酶(nNOS)。结合和沉淀DNA,可用于DNA结合蛋白纯化。激活T4聚核苷酸激酶活性。Caution
如果需要无菌溶液,则应采用无菌过滤而非高压灭菌。时间一长,亚精胺将发生脱氨基作用;溶液应采用冷冻保存。通常需要配制新溶液。