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糊精琼脂糖凝胶HP [MBP标签蛋白纯化树脂],Dextrin Sepharose High Performance MBP-tagged protein purification resin

Dextrin Sepharose High Performance 是一种强大且稳定的亲和树脂,用于纯化标记有麦芽糖结合蛋白 (MBP) 的蛋白质。28935598;28935597
货号(SKU): 28935598
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€1481.53

Dextrin Sepharose High Performance is a robust and stable affinity resin for purification of proteins tagged with maltose binding protein (MBP).

快速、一步纯化 MBP 标记的蛋白质。
窄峰中的高分辨率纯化和洗脱,最大限度地减少了进一步浓缩步骤的需要。
可用于重复纯化:可使用 0.5 M NaOH 轻松再生。
生理条件和温和的洗脱保留了目标蛋白的活性。
纯化用麦芽糖结合蛋白标记的蛋白质
重组蛋白带有 MBP 标签,便于检测、分离和纯化程序。 此外,通常选择这种亲和标签是因为它能够提高融合蛋白的表达水平和溶解度。

重组蛋白的纯化是在生理条件下进行的,结合麦芽糖的温和洗脱,保留了目标蛋白的活性。

  • Fast, one-step purification of MBP-tagged proteins.
  • High-resolution purification and elution in narrow peaks, minimizing the need for further concentration steps.
  • Can be used for repeated purification: can be easily regenerated using 0.5 M NaOH.
  • Physiological conditions and mild elution preserve target protein activity.

Purifying proteins tagged with maltose binding protein

Recombinant proteins are engineered with an MBP-tag to facilitate detection, isolation, and purification procedures. In addition, this affinity tag is often chosen due to its ability to increase the expression level and solubility of the fusion protein.

Purification of recombinant proteins is done under physiological conditions, which together with mild elution by maltose, preserves the activity of the target protein.

Dextrin Sepharose High Performance MBP resin

The small, evenly sized beads ensure that tagged proteins elute in narrow peaks, thus minimizing the need for further concentration steps. The resin is easily regenerated using 0.5 M NaOH, allowing the same column to be used to purify different proteins.

Successful use in published research

Research and applications using MBP resin include:

Compatible empty columns

Cytiva's solutions include a variety of empty columns. Dextrin Sepharose High Performance resin is compatible with Tricorn, XK, and HiScale column lines.

Convenient prepacked columns

This resin is also available as prepacked MBP-Trap columns. These HiTrap format columns can be used with a syringe, a pump, or a chromatography system.

Other Cytiva products for affinity purification

Looking for a different affinity resin? Let Cytiva help you find the right product to purify your tagged recombinant protein.

 

ParameterDextrin Sepharose High Performance, 100 mL
LigandDextrin 
Particle size, d50V1~34 µm 
MatrixHighly cross-linked agarose, 6% 
Binding Capacity/ml Chromatography MediumApprox. 7 mg MBP- ∆Sal/ml resin Approx. 16 mg MBP- βGal/ml resin 
pH stability, CIP22–13 
Flow velocity< < 300 cm/h 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityStable in commonly used aqueous buffers, 0.5 M NaOH (regeneration and cleaning) 
Certificate of AnalysisYes 
Pack size100 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32