Recombinant proteins are engineered with an MBP-tag to facilitate detection, isolation, and purification procedures. In addition, this affinity tag is often chosen due to its ability to increase the expression level and solubility of the fusion protein.
Purification of recombinant proteins is done under physiological conditions, which together with mild elution by maltose, preserves the activity of the target protein.
Dextrin Sepharose High Performance MBP resin
The small, evenly sized beads ensure that tagged proteins elute in narrow peaks, thus minimizing the need for further concentration steps. The resin is easily regenerated using 0.5 M NaOH, allowing the same column to be used to purify different proteins.
Successful use in published research
Research and applications using MBP resin include:
Compatible empty columns
Cytiva's solutions include a variety of empty columns. Dextrin Sepharose High Performance resin is compatible with Tricorn, XK, and HiScale column lines.
Convenient prepacked columns
This resin is also available as prepacked MBP-Trap columns. These HiTrap format columns can be used with a syringe, a pump, or a chromatography system.
Other Cytiva products for affinity purification
Looking for a different affinity resin? Let Cytiva help you find the right product to purify your tagged recombinant protein.
|Parameter||Dextrin Sepharose High Performance, 100 mL|
|Particle size, d50V1||~34 µm |
|Matrix||Highly cross-linked agarose, 6% |
|Binding Capacity/ml Chromatography Medium||Approx. 7 mg MBP- ∆Sal/ml resin Approx. 16 mg MBP- βGal/ml resin |
|pH stability, CIP2||2–13 |
|Flow velocity||< < 300 cm/h |
|Storage||2 to 8°C, 20% Ethanol |
|Chemical stability||Stable in commonly used aqueous buffers, 0.5 M NaOH (regeneration and cleaning) |
|Certificate of Analysis||Yes |
|Pack size||100 mL |