General description
Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]
用于琼脂糖或聚丙烯酰胺凝胶中核酸或蛋白质电泳的示踪染料。包装
5, 10, 25 g in glass bottle
Reconstitution
使用含0.015%溴酚蓝的标准配方,可制备6x凝胶上样缓冲液。