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人纤维母细胞生长因子-碱性,Fibroblast Growth Factor-Basic human [hBFGF, FGF2];FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

FGF-碱性,重组,在大肠杆菌中表达,适用于细胞培养;人成纤维细胞生长因子-碱性
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): F0291
CAS号: 106096-93-9
MDL号: MFCD00081382
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¥3,550.00

说明

成纤维细胞生长因子-碱性 (FGF-Basic) 存在于基底膜和亚内皮细胞外基质中。 FGF-Basic 在胚胎发育早期被诱导,胚胎干细胞 (ESC) 和谱系定义的干细胞的多能性和自我更新是必要的(但不充分); 其中它起到支持增殖和抑制分化的作用。 hESC 的自我更新依赖于激活素/Nodal/Smad2,3 分支的激活和 TGFβ 家族细胞信号传导程序的 BMP/GDF/Smad5 分支的抑制。 FGF-Basic 是多能性节点支持的能力因素。 Activin-A 是维持干性所必需且足够的。 它在人 ES 细胞中诱导 Nodal 和 FGF-Basic 的表达。

成纤维细胞生长因子-碱性 (FGF-Basic) 是维持培养中的人类胚胎干细胞和各种其他干细胞系,如间充质干 (基质) 细胞 (MSC) 所必需的。

一般描述

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
 

应用

Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).
 

外形

Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
 

分析说明

The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
 
 

属性

质量水平

200

生物来源

human

重组

expressed in E. coli

测定

≥97% (SDS-PAGE)

形式

lyophilized powder

效能

0.05-0.6 ng/mL

分子量

16.0 kDa

储存条件

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

杂质

≤1.00 EU/μg

颜色

white to faint yellow cast

溶解性

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt登记号

储存温度

−20°C

Gene Information

human ... FGF2 (2257)

 

安全信息

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 55 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 8 | 香港与北京 ≥ 10