亲和素 [亲合素]，Avidin from egg white；recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

General description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians.^[1][2] Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity.^[2] Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants.^[1] Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.^[3][4]

Application

Avidin forms an extremely strong complex with d-biotin (K_d ~ 10^-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.

亲和素蛋白用于一项分析中，该分析使用官能化氙作用检测生物素-亲和素结合的生物传感器。使用80nmol的鸡蛋白。

包装

5 mg in glass bottle

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Preparation Note

亲和纯化

性质

安全信息