Capto L combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.
Using this protein L resin
Capto L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, such as PreDictor™ plates, PreDictor™ RoboColumn™, and HiScreen™ prepacked columns.
Successful use in published research
Capto L is used in applications requiring the purification of antibody fragments through protein L affinity chromatography.
Compatible empty columns – research scale
Capto L resin is compatible with empty PD gravity flow, Tricorn, XK, and HiScale columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns.
Convenient prepacked columns – research scale
Capto L resin is available in the following formats:
- HiTrap™ Protein L: convenient for easy use with a syringe, peristaltic pump, or chromatography system
- HiScreen Capto™ L prepacked column, for method and process development
- PreDictor™ Capto™ L 96-well plate, for high-throughput screening or process development
Convenient prepacked columns – bioprocess scale
Prepacked ReadyToProcess columns are available, eliminating the need for column packing and cleaning validation.
|Parameter||Capto L, 1 L|
|Chromatography technique||Antibody affinity chromatography |
|BioProcess resin||Yes |
|Ligand||Recombinant protein L (E. coli), mammalian free |
|Particle size, d50V1||~85 µm |
|Matrix||Rigid, highly cross-linked agarose |
|pH stability, operational2||2–10 |
|Flow velocity3||< 500 cm/h |
|Coupling Chemistry||Epoxy activation |
|Storage||2°C to 8°C in 20% ethanol |
|Recommended Cleaning-in-Place Reagent||15 mM NaOH |
|Pack size||1 L |