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Capto L抗体片段纯化树脂,Capto L antibody fragment purification resin

Capto L 抗体片段纯化树脂专为抗体片段纯化而设计,当片段包含 kappa 轻链时。17547802;17547806;17547804;17547801;17547803;17547805
货号(SKU): 17547803
增值税发票 √ 免费送货 √ 订货电话:010-68689484
$1,237.68

Capto L resin is designed for antibody fragment purification, when the fragment contains the kappa light chain.

适用于捕获各种抗体片段,例如 Fab、dAb 和单链片段变量 (scFv)。
高结合能力、低配体泄漏和对广泛抗体片段的选择性减少了处理时间和树脂用量。
实现抗体片段纯化的平台方法。
由于刚性矩阵,具有出色的生产率、工艺经济性和工艺设计灵活性。
亲水性基础基质确保洗脱池中非特异性结合水平低,宿主细胞来源的杂质水平低。
满足工业对供应安全、稳健性能和监管支持的需求。
用于抗体片段纯化的亲和树脂
Capto L 将刚性、高流动性琼脂糖基质与免疫球蛋白结合重组蛋白 L 配体结合,后者对抗体 kappa 轻链的可变区具有很强的亲和力。

对多种抗体片段的高结合能力和亲和力使该树脂成为捕获含有κ轻链的抗体片段的绝佳平台。常见用途包括域抗体 (dAb)、scFv 和 Fab 纯化。

 

  • Suitable for the capture of a wide range of antibody fragments such as Fabs, dAbs, and single-chain fragment variable (scFv) .
  • High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
  • Enables a platform approach to antibody fragment purification.
  • Excellent productivity, process economy, and process design flexibility due to rigid matrix.
  • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Affinity resin for antibody fragment purification

Capto L combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.

The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.

Using this protein L resin

Capto L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.

Streamlined scale up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, such as PreDictor™ plates, PreDictor™ RoboColumn™, and HiScreen™ prepacked columns.

Successful use in published research

Capto L is used in applications requiring the purification of antibody fragments through protein L affinity chromatography.

Compatible empty columns – research scale

Capto L resin is compatible with empty PD gravity flow, Tricorn, XK, and HiScale columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns.

Convenient prepacked columns – research scale

Capto L resin is available in the following formats:

  • HiTrap™ Protein L: convenient for easy use with a syringe, peristaltic pump, or chromatography system
  • HiScreen Capto™ L prepacked column, for method and process development
  • PreDictor™ Capto™ L 96-well plate, for high-throughput screening or process development

Convenient prepacked columns – bioprocess scale

Prepacked ReadyToProcess columns are available, eliminating the need for column packing and cleaning validation.

 

ParameterCapto L, 1 L
Chromatography techniqueAntibody affinity chromatography 
BioProcess resinYes 
LigandRecombinant protein L (E. coli), mammalian free 
Particle size, d50V1~85 µm 
MatrixRigid, highly cross-linked agarose 
pH stability, operational22–10 
Flow velocity3< 500 cm/h 
Coupling ChemistryEpoxy activation 
Storage2°C to 8°C in 20% ethanol 
Recommended Cleaning-in-Place Reagent15 mM NaOH 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3At bed height 20 cm
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16