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丙酮酸激酶M2,Pyruvate kinase M2 Active from mouse [PKM2, PK3, M2-PK tumor];recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

别名: M2-PK tumor, OPA-interacting protein 3 (OIP-3), Pyruvate kinase 3 (PK3), Pyruvate kinase, muscle, Thyroid hormone-binding protein 1 (THBP1) NACRES: NA.32
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRP0189
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¥8,244.76

说明

丙酮酸激酶M2(PKM2),也称为丙酮酸激酶3(PK3),是丙酮酸激酶的一种亚型,在一些分化的组织中表达,如肺、脂肪、视网膜和胰岛,以及正常增殖细胞和胚胎细胞。PKM2/pk3由定位于小鼠9号染色体的基因编码。
小鼠丙酮酸激酶M2(PKM2)(基因库入藏号NM_011099),氨基酸2端,N末端His标签,MW = 58kDa,在大肠杆菌表达系统中表达。

在大多数癌细胞中观察到PKM2的表达升高。因此,这种蛋白质可以被认为是癌症的有效治疗靶点。PKM2在过量的细胞内活性氧(ROS)的释放中起重要作用,否则会损害细胞成分并降低细胞活力。PKM2的蛋白激酶活性参与基因表达和肿瘤发生的调节。PKM2是一种重要的限速酶,参与糖酵解的最后一步,在整个癌症进展过程中的代谢重编程中起着至关重要的作用。

一般描述

Pyruvate kinase M2 (PKM2), also known as pyruvate kinase 3 (PK3), is an isoform of pyruvate kinase that is expressed in some differentiated tissues, such as lung, fat, retina, and pancreatic islets, as well as in normal proliferating cells and embryonic cells.[1] PKM2/pk3 is encoded by the gene mapped to mouse chromosome 9.[2]
Murine Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_011099), amino acids 2-end, with N-terminal His tag, MW = 58kDa, expressed in an Escherichia coli expression system.
 

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
 

生化/生理作用

Elevated expression of PKM2 is observed in most of the cancer cells. Hence, this protein can be considered as a potent therapeutic target for cancers.[3][1] PKM2 plays an important role in the discharge of excessive intracellular reactive oxygen species (ROS), which otherwise damage cellular components and decrease cell viability. Protein kinase activity of PKM2 is involved in the regulation of gene expression and tumorigenesis. PKM2 is an important rate-limiting enzyme involved in final step of glycolysis and it has an essential role in metabolic reprogramming throughout cancer progression.[1][3]
 

单位定义

One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37°C
 

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
 

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
 
 

属性

生物来源

mouse

重组

expressed in E. coli

测定

≥90% (SDS-PAGE)

形式

aqueous solution

分子量

58 kDa

包装

pkg of 20 μg

储存条件

avoid repeated freeze/thaw cycles

浓度

>0.02 mg/mL

NCBI登记号

UniProt登记号

运输

dry ice

储存温度

−70°C

Gene Information

mouse ... PKM2(18746)

 

安全信息

监管信息

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商品规格
属性名称属性值
储存温度 Storage temp.-70°C超低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32