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三(羟甲基)甲基甘氨酸,Tricine;≥99% (titration)

别名:Tricine缓冲液;N-三(羟甲基)甲基甘氨酸;三羟甲基甲基甘氨酸;N-[Tris(hydroxymethyl)methyl]glycine

线性分子式: (HOCH2)3CNHCH2CO2H CAS号: 5704-04-1 分子量: 179.17 Beilstein: 1937804 EC 号: 227-193-6 MDL编号: MFCD00004277 PubChem化学物质编号: 24899884 NACRES: NA.25
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): T0377
CAS号: 5704-04-1
MDL号: MFCD00004277
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¥443.99

说明

一般描述

Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels.[1]
 

应用

Tricine has been used:
  • to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures[2]
  • as a component of buffer A for the homogenization of samples like Caenorhabditis elegansDrosophila, and plants[3]
  • as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay[4]
用于分离低分子量的肽的缓冲剂组分。
 
 

属性

质量水平

测定

≥99% (titration)

形式

crystalline powder

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

溶解性

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

 

安全信息

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16