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ZCL278,CDC42 Inhibitor III;≥98% (HPLC)

别名:CDC42 GTPase Inhibitor III;2-(4-溴-2-氯苯氧基)-N-((4-(N-(4,6-二甲基嘧啶-2-基)氨磺酰基)苯基)氨基硫代甲酰基)乙酰胺;4-(3-(2-(4-Bromo-2-chloro-phenoxy)-acetyl)-thioureido)-N-(4,6-dimethyl-pyrimidin-2-yl)-benzenesulfonamide, 2-(4-Bromo-2-chlorophenoxy)-N-((4-((4,6-dimethyl-2-pyrimidinyl)sulfamoyl)phenyl)carbamothioyl)acetamide

CAS号 587841-73-4 经验分子式(希尔表示法) C21H19BrClN5O4S2 分子量 584.89
制造商品牌: BSZH 科学公司
货号(SKU): C06116
CAS号: 587841-73-4
MDL号: MFCD03623090
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¥1,216.00

产品描述

一种选择性的Cdc42抑制剂,Kd为11.4 μM。

General description

A cell-permeable, non-cytotoxic, aqueous soluble (181 µM in PBS, pH 7.4) pyrimidinylthiourea that targets Cdc42 GEF ITSN/intersectin and adjacent GTP/GDP binding site and effectively inhibits EGF-stimulated Cdc42 activity (1 h pretreatment; 50 µM) and Cdc42-dependent microspike/filopodia formation in serum-starved Swiss 3T3 fibroblast cultures without affecting RhoA- or Rac1-dependent functions. Short-term ZCL278 exposure in primary murine neonatal cortical neurons is shown to dramatically abolish neurite branching (50 µM).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

A cell-permeable, non-cytotoxic, aqueous soluble (181 µM in PBS, pH 7.4, containing 0.33% DMSO) pyrimidinylthiourea compound that targets Cdc42 GEF ITSN/intersectin and adjacent GTP/GDP binding site (Kd = 6.4 and 11.4 µM by fluorescence titration and SPR, respectively) and effectively inhibits EGF-stimulated Cdc42 activity (by 100% with 1 h pretreatment of 50 µM ZCL278) and Cdc42-dependent microspike/filopodia formation in serum-starved Swiss 3T3 fibroblast cultures without affecting RhoA-dependent cellular processes or Rac1-dependent lamellipodia formation. Short-term ZCL278 exposure in 5 d-cultured primary neonatal cortical neurons from 1 d postnatal mice is shown to dramatically abolish neurite branching (Ave branches per neurite = 21, 7.8, and 6.4, respectively, with 0, 5, and 10 min exposure to 50 µM ZCL278).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
CDC42

Reversible: yes

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze 9-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Friesland, A., et al. 2013. Proc. Natl. Acad. Sci. USA110, 1261.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

性质

assay  ≥98% (HPLC)
form  solid
storage condition  OK to freeze
 protect from light
storage conditions  -20 ℃
color  light beige
solubility  DMSO: 50 mg/mL


安全信息

RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16