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Strep-Tactin® XT琼脂糖凝胶层析树脂,Strep-Tactin® XT Sepharose chromatography resin

用于纯化带有 Strep-tag® II 和 Twin-Strep-tag® 标签的重组蛋白的层析树脂。29401326;29401324
货号(SKU): 29401326
增值税发票 √ 免费送货 √ 订货电话:010-68689484
€1159.73

Chromatography resin for purification of recombinant proteins tagged with a Strep-tag® II and Twin-Strep-tag®.

一种用于纯化标记有 Strep-tag II 和 Twin-Strep-tag 的重组蛋白的层析树脂。 这些蛋白质与固定的 Strep-Tactin XT 配体非常特异性地结合,提供高纯度的目标蛋白质。

高纯度 Strep-tag II 和 Twin-Strep-tag 重组蛋白。
快速纯化,简单洗脱。
生理条件和温和的洗脱保留了目标蛋白的活性。
快速和容易的再生。
与多种洗涤剂、变性剂和其他添加剂兼容。
提供 10 和 50 mL 实验室包装的散装树脂,并预装在 1 和 5 mL StrepTrap XT 色谱柱中以方便使用。
Strep-Tactin XT Sepharose 包含最新的 Strep-Tactin Sepharose 配体 XT,与之前的产品 StrepTactin Sepharose High Performance (HP) 相比,它具有更高的亲和力,从而导致更高的产量和更少的有价值目标蛋白的损失。 Strep-Tactin XT Sepharose 还可在变性条件下进行纯化。

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilized Strep-Tactin XT ligand giving highly pure target protein.

  • Highly pure Strep-tag II and Twin-Strep-tag recombinant proteins.
  • Fast purifications with simple elution.
  • Physiological conditions and mild elution preserve target protein activity.
  • Fast and easy regeneration.
  • Compatible with a wide range of detergents, denaturants, and other additives.
  • Available in 10 and 50 mL lab packs of bulk resin and prepacked in 1 and 5 mL StrepTrap XT chromatography columns for convenience.

Strep-Tactin XT Sepharose contains the latest Strep-Tactin ligand, XT, which provides higher affinity, resulting in higher yields and lower loss of valuable target protein than with the predecessor product, StrepTactin Sepharose High Performance (HP). Strep-Tactin XT Sepharose also enables purification under denaturing conditions.

 

ParameterStrep-Tactin XT Sepharose, 50 mL
LigandStrep-Tactin XT 
Particle size, d50V134 µm 
MatrixRigid, cross-linked agarose, spherical 
Dynamic binding capacity, QB102~ 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin 
Ligand density~ 5 mg/mL resin 
ApplicationPurification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag 
pH stability, operational3pH 6 to 10 
Recommended maximum operational flow velocity300 cm/h 
Storage2°C to 8°C in 20% ethanol 
Chemical stabilityStable in all commonly used aqueous buffers 
Pack size50 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2Binding capacity is protein dependent. Dynamic binding capacity (DBC) is defined as milligram of protein applied per milliliter of resin at the point where the concentration of protein in the column eluent reaches a value of 10% of the concentration in the sample. DBC was tested here at a flow rate of 1 mL/min in a 1 mL StrepTrap XT column (1 min residence time) for GAPDH Twin-Strep-tag (Mᵣ 39 400/subunit) and GAPDH Strep-tag II (Mᵣ 37 400/subunit) in 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8.
  • 3pH range where resin can be operated without significant change in function.
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32