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sTNF-receptor Type I human,[TNF-R55, TNFAR, TNFRSF1A];recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

别名:Soluble Tumor Necrosis Factor type I, TNF-R55, TNFAR, TNFRSF1A;NACRES NA.32
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRP3162
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¥3,574.00

产品描述

General description

TNFR1 (tumor necrosis factor receptor 1) gene is localized to human chromosome 12p13, and encodes a 55kDa receptor showing a wide range of tissue expression. This receptor belongs to the family of receptors which contain repeating cysteine-rich extracellular motifs. TNFR1 contains a protein-protein interacting domain called death domain containing ∼70 amino acids. It also contains a silencer of death domains (SODD), a 60kDa domain, which inhibits ligand-independent self-association of TNFR1.[1] TNFR1 is a member of the TNFR family, which are type I transmembrane proteins.[2]
Recombinant human sTNFR1 is an 18.3 kDa protein (162 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein.

Biochem/physiol Actions

TNFR1 (tumor necrosis factor receptor 1) is involved in inflammatory responses, and the cleavage of the membrane form leads to production of soluble TNFR1. The soluble form competes with the membrane-bound form, and this leads to a reduction in the severity of the inflammatory responses. Mutations in this gene resulting in the disruption of conserved extracellular disulfide bonds, leads to lower levels of soluble TNFR1 forms, which is linked with autosomal dominant periodic fever syndromes, characterized by unexplained recurrent fevers and severe localized inflammation.[1] Studies in two independent community-based elderly cohorts show that sTNFR1 is linked with increased mortality in cardiovascular and cancer cases.[3]

Sequence

MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN

Physical form

Lyophilized from 10 mM Tris, pH 8.0 + 20 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris, pH 8.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

性质

biological source  human
recombinant  expressed in E. coli
assay  ≥98% (HPLC)
 ≥98% (SDS-PAGE)
form  lyophilized
potency  0.25 ng/mL ED50
mol wt  18.3 kDa
packaging  pkg of 20 μg
application(s)  cell culture | mammalian: suitable
impurities  <0.1 EU/μg endotoxin, tested
color  white
UniProt accession no.  P19438
shipped in  wet ice
storage temp.  −20°C
Gene Information  human ... TNFRSF1A(7132)


安全信息

危害码 (欧洲) Xi
风险声明 (欧洲) 36/37/38
安全声明 (欧洲) 26
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16
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