SP Sepharose XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
- High loading capacities give more productive capture from clarified feedstocks.
- Strong cation exchanger designed for capture steps
- High chemical stability for effective CIP/sanitization
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
SP Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established SP Sepharose Fast Flow resin. Dextran chains are covalently coupled to the agarose matrix and modified with sulphopropyl (SP) strong cation exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates
With its high loading capacities and excellent physical and chemical stability, SP Sepharose XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.
As member of the BioProcess resins range, SP Sepharose XL meets industrial demands with security of supply and comprehensive technical and regulatory support.
|Parameter||SP Sepharose XL|
|BioProcess resin||Yes |
|Particle size, d50V1||~90 μm |
|Ion Exchanger Type||Strong cation exchanger |
|Matrix||6% cross-linked agarose with dextran surface extenders |
|Particle Size||45 μm-165 μm |
|Ligand Coupling Method||Dextran extenders through ether bonds |
|Ionic Capacity||0.18-0.25 mmol H+/ml medium |
|pH stability, operational2||4–13 |
|pH stability, CIP3||3–14 |
|Pressure/Flow Specification||300-500 cm/h |
|Storage||4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate |
|Chemical stability||Commonly used buffers, nonionic detergents, 1 M NaOH, 6 M guanidine hydrochloride |
|Certificate of Analysis||Yes |