性质
SP Sepharose High Performance is a strong cation exchange BioProcess resin with high resolution designed for intermediate and polishing steps in downstream processing
- Strong cation exchanger for intermediate and final polishing steps
- High resolution, high capacity separation with high recovery
- High chemical stability for effective CIP/sanitization
- BioProcess resin supported for industrial applications and well-established in approved processes
SP Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. SP Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide.
SP Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, SP Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support.
规格
Parameter | SP Sepharose High Performance |
---|
BioProcess resin | Yes |
Ligand | Sulphopropyl |
Particle size, d50V1 | ~34 µm |
Ion Exchanger Type | Strong cation exchanger |
Matrix | 6% cross-linked agarose |
Particle Size | 24 µm-44 µm |
Ligand Coupling Method | Ether linkages |
Ionic Capacity | 0.15-0.20 mmol H+/ml |
pH stability, operational2 | 4–13 |
pH stability, CIP3 | 3–14 |
Pressure/Flow Specification | 150 cm/h (at < 3 bar, 25°C, 10 cm bed height) |
Exclusion limit (Mr) (globular proteins) | ~4 × 10⁶ |
Storage | 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate |
Chemical stability4 | Stable in commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS |
Charged Groups | - SO₃- |
Certificate of Analysis | Yes |