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SP琼脂糖凝胶FF [高流速]，SP Sepharose Fast Flow [GE Cytiva]

SP Sepharose Fast Flow是用于快速蛋白质纯化的磺丙基（SP）强阳离子交换色谱树脂。订货号：17072910；17072960；17072901；17072905；17072904；17-0729-10；17-0729-60；17-0729-01；17-0729-05；17-0729-04

品牌: GE 生命科学 [思拓凡 Cytiva]

货号（SKU）: 17072901

MDL号: MFCD00148494

增值税发票 √ 免费货运 [顺丰联邦] √

目录价.SKU-Variant

25mL
300mL [+¥5,895.00]

¥1,230.00

数量:

产品描述

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.

SP 琼脂糖凝胶 ^® 用于蛋白质色谱、离子交换色谱和阳离子交换介质。SP琼脂糖凝胶^® 已被用于姜科植物根茎中抑制蛋白和多肽的研究以及大豆碱性 7S 球蛋白亚基异质性和分子进化的研究。此外，SP 琼脂糖凝胶 ^® 还有助于推动壳寡糖制备的工业应用。

性质

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.

Well established strong cation exchanger.
Based on Sepharose Fast Flow ion exchange resin.
High chemical stability enables proven CIP and sanitization protocols.
Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Streamlined scale-up

SP Sepharose Fast Flow uses the Sepharose Fast Flow ion exchange resin, which is well established in industrial downstream processing. The highly crosslinked agarose resin enables high flow rates and high productivity.

Composed of crosslinked 6% agarose beads with sulphopropyl (SP) strong cation exchange groups, this resin has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.

Scale-up with these SP media (resins) is straightforward. Scale-up is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, PreDictor RoboColumns™, and HiTrap columns.

For even faster flow rates and better binding capacity in scale-up, Capto S and other Capto resins are a good option.

Successful use – research scale

SP Sepharose Fast Flow media (resins) are used in a variety of research applications, including:

Two-step ion exchange chromatography of human calprotectin from neutrophils for structural studies
Purification of APT1 from rat liver cytosol
Purification of a manganese peroxidase from Panaeolus sphinctrinus

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns for research scale. SP Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. SP Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked columns – research scale

This strong cation exchange resin is available in the following prepacked formats:

HiTrap SP FF columns – convenient for easy use with a syringe, peristaltic pump, or chromatography system such as ÄKTA system
HiScreen SP FF columns for scale up and process development.
HiPrep SP FF columns for convenient scale-up.

Prepacked columns – bioprocess scale

Prepacked ReadyToProcess columns are available for bioprocess applications.

规格

Parameter	SP Sepharose Fast Flow
BioProcess resin	Yes
Ligand	Sulphopropyl
Particle size, d50V1	~90 µm
Ion Exchanger Type	Strong cation exchanger
Matrix	cross-linked agarose, 6%, spherical
Particle Size	45 µm-165 µm
Ionic Capacity	0.18-0.25 mmol H+/ml medium
pH stability, operational2	4–13
pH stability, CIP3	3–14
pH ligand fully charged4	Entire pH range
Pressure/Flow Specification	400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Exclusion limit (Mr) (globular proteins)	~4 × 10⁶
Storage	4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
Chemical stability5	Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

1Median particle size of the cumulative volume distribution.
2pH range where resin can be operated without significant change in function.
3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
4pH range where ligand is fully charged.
51 M NaOH and acetic acid should be used only for cleaning purposes.

安全信息

个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

RIDADR NONH for all modes of transport

WGK德国 3

商品规格

属性名称	属性值
储存温度 Storage temp.	常温阴凉避光
全球实时库存 Availability √	美国St. Louis ≥ 190 \| 欧洲Eur. ≥ 66 \| 東京Tokyo ≥ 82 \| 香港与北京 ≥ 55

商品标签

SP琼脂糖凝胶FF [高流速]，SP Sepharose Fast Flow [GE Cytiva]

产品描述

性质

Streamlined scale-up

Successful use – research scale

Compatible empty columns – research scale

Compatible empty columns – bioprocess scale

Prepacked columns – research scale

Prepacked columns – bioprocess scale

规格

安全信息

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