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SP琼脂糖凝胶FF [高流速],SP Sepharose Fast Flow [GE Cytiva]

SP Sepharose Fast Flow是用于快速蛋白质纯化的磺丙基(SP)强阳离子交换色谱树脂。订货号:17072910;17072960;17072901;17072905;17072904;17-0729-10;17-0729-60;17-0729-01;17-0729-05;17-0729-04
货号(SKU): 17072901
MDL号: MFCD00148494
增值税发票 √ 免费货运 [顺丰 联邦] √
¥884.00

产品描述

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.

SP 琼脂糖凝胶 ® 用于蛋白质色谱、离子交换色谱和阳离子交换介质。SP琼脂糖凝胶® 已被用于姜科植物根茎中抑制蛋白和多肽的研究以及大豆碱性 7S 球蛋白亚基异质性和分子进化的研究。此外,SP 琼脂糖凝胶 ® 还有助于推动壳寡糖制备的工业应用。

性质

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.

  • Well established strong cation exchanger.
  • Based on Sepharose Fast Flow ion exchange resin.
  • High chemical stability enables proven CIP and sanitization protocols.
  • Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Streamlined scale-up

SP Sepharose Fast Flow uses the Sepharose Fast Flow ion exchange resin, which is well established in industrial downstream processing. The highly crosslinked agarose resin enables high flow rates and high productivity.

Composed of crosslinked 6% agarose beads with sulphopropyl (SP) strong cation exchange groups, this resin has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.

Scale-up with these SP media (resins) is straightforward. Scale-up is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, PreDictor RoboColumns™, and HiTrap columns.

For even faster flow rates and better binding capacity in scale-up, Capto S and other Capto resins are a good option.

Successful use – research scale

SP Sepharose Fast Flow media (resins) are used in a variety of research applications, including:

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns for research scale. SP Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. SP Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked columns – research scale

This strong cation exchange resin is available in the following prepacked formats:

  • HiTrap SP FF columns – convenient for easy use with a syringe, peristaltic pump, or chromatography system such as ÄKTA system
  • HiScreen SP FF columns for scale up and process development.
  • HiPrep SP FF columns for convenient scale-up.

Prepacked columns – bioprocess scale

Prepacked ReadyToProcess columns are available for bioprocess applications.

规格

ParameterSP Sepharose Fast Flow
BioProcess resinYes 
LigandSulphopropyl 
Particle size, d50V1~90 µm 
Ion Exchanger TypeStrong cation exchanger 
Matrixcross-linked agarose, 6%, spherical 
Particle Size45 µm-165 µm 
Ionic Capacity0.18-0.25 mmol H+/ml medium 
pH stability, operational24–13 
pH stability, CIP33–14 
pH ligand fully charged4Entire pH range 
Pressure/Flow Specification400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm 
Exclusion limit (Mr) (globular proteins)~4 × 10⁶ 
Storage4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate 
Chemical stability5Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
  • 4pH range where ligand is fully charged.
  • 51 M NaOH and acetic acid should be used only for cleaning purposes.


安全信息

个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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