SP Sepharose Big Beads is a strong cation exchanger designed for industrial applications in the capture step of large volumes of feed or viscous feedstocks
- Strong cation exchanger for capture steps handling large volumes of feed.
- Large agarose beads (100-300 µm) permit capture from viscous feedstocks at high flow velocities
- High chemical resistance for effective cleaning-in-place (CIP)
- BioProcess resin supported for industrial applications and well-established in approved processes
SP Sepharose Big Beads is composed of large crosslinked agarose beads (100-300 µm) modified with sulphonate (SP) strong cation exchange groups. It is designed for industrial applications and the large particle size and physical stability of the base matrix ensure maintained performance and low back-pressures even with viscous samples.
As member of the BioProcess resins range, SP Sepharose Big Beads meets industrial demands with security of supply and comprehensive technical and regulatory support.
|Parameter||SP Sepharose Big Beads|
|BioProcess resin||Yes |
|Particle size, d50V1||~200 µm |
|Ion Exchanger Type||Strong cation exchanger |
|Matrix||6% cross-linked agarose |
|Particle Size||100 µm-300 µm |
|Ligand Coupling Method||Ether bonds |
|Ionic Capacity||0.18-0.25 mmol H+/ml medium |
|pH stability, operational2||4–12 |
|pH stability, CIP3||3–14 |
|Pressure/Flow Specification||1200-1800 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
|Exclusion limit (Mr) (globular proteins)||~4 × 10⁶ |
|Storage||4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate |
|Chemical stability||Stable in commonly used aqeous buffers, 1M NaOH, 70% ethanol, organic solvents |
|Certificate of Analysis||Yes |