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RPMI-1640培养基,RPMI-1640 Medium;With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

含L-谷氨酰胺,不含碳酸氢钠,粉末型1640,适合细胞培养;别名:Roswell Park Memorial Institute 1640 medium; MDL编号: MFCD00217820 NACRES: NA.75
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): R6504
MDL号: MFCD00217820
增值税发票 √ 免费送货 √ 订货电话:010-68689484
¥327.70

说明

一般描述

洛斯维·帕克纪念研究所培养基通常被称为PRMI。 是一种用于细胞培养和组织培养的培养基。该培养基含有磷酸,适用于含有5%二氧化碳的气体。它可用于人淋巴细胞生长。
 

应用

PRMI-1640培养基可用于以下应用:
  • 用于细胞培养培养基(比如RPMI1640培养基加上其他组分可以用来培养小鼠胸腺瘤EL-4)
  • 用于脾细胞分枝杆菌感染(在无抗生素PRMI1640培养基)
  • 用于细胞分离和培养
  • 用于淋巴细胞分离和培养,ELISPOT分析,体外增生实验培养基(组分之一)
  • gp39基因表达和CD40-免疫球蛋白结合分析培养基
  • 用于制备抗真菌剂
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
 

数量

按照每升培养基10.4克粉末配制。
 

重悬

补充2.0 g/L碳酸氢钠。
 
 

属性

无菌性

sterile

形式

powder

technique(s)

cell culture | mammalian: suitable

组分

L-glutamine: yes
HEPES: no
phenol red: yes
NaHCO3: no
sodium pyruvate: no

运输

ambient

储存温度

2-8°C

 

安全信息

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

监管信息

动植物来源培养基
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16
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