RPMI-1640培养基，RPMI-1640 Medium；With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。通过对McCoy′s 5A培养基进行修改，它被配制用于在悬浮培养中支持淋巴母细胞，但一直以来它也被证明可以支持各种不同锚定依赖的细胞。

 

RPMI-1640培养基已被用作维持细胞系的培养基并作为不同细胞的培养基。

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.