RPMI-1640培养基，RPMI-1640 Medium；Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

RPMI-1640培养基被用于以下应用：

• 用于细胞培养基：在补充有其他组分的RPMI 1640培养基中培养小鼠胸腺瘤EL-4细胞
• 用于脾细胞的分枝杆菌感染（在无抗生素的RPMI 1640培养基中）
• 用于细胞分离和培养
• 用于淋巴细胞分离和培养、ELISPOT测定、间接体内增殖测定所用的培养基中（作为组分之一）
• 用于gp39基因表达和CD40-免疫球蛋白结合试验所用的培养基中
• 用于制备抗真菌剂

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

 

按照每升培养基10.4克粉末配制。

 

补充2.0 g/L碳酸氢钠。

 

酚红会干扰一些细胞在克隆密度下的生长。该培养基适用于干细胞培养或低密度的细胞培养。也推荐用于体外诊断。