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RPMI-1640培养基,RPMI-1640 Medium;HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture

HEPES修饰,含25 mM HEPES,不含L-谷氨酰胺。液体,无菌过滤,适用于细胞培养;别名:Roswell Park Memorial Institute 1640 medium; MDL编号: MFCD00217820 NACRES: NA.75
货号(SKU): R5886
MDL号: MFCD00217820
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¥194.36

说明

该 RPMI-1640培养基添加了HEPES。 HEPES是Good的生理缓冲液,与碳酸氢盐缓冲液相比,它有助于提供更稳定的培养基pH值。

RPMI 1640培养基由Moore和他的同事于1966年在罗斯威尔公园纪念研究所开发。 作为McCoy 5A培养基的改良版,它被配制用于支持悬浮培养中的淋巴母细胞,但后来被证明支持多种依赖贴壁的细胞。 最初打算与血清补充剂一起使用,RPMI 1640已被证明在没有血清的情况下支持多种细胞系。 它还广泛用于融合方案和杂交细胞的生长。 该培养基适用于培养人类正常和肿瘤性白细胞。

一般描述

This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.
 

应用

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
 
 

属性

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

pH值(酸碱度)

>7.2

组分

L-glutamine: no
NaHCO3: yes
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes

运输

ambient

储存温度

2-8°C

 

安全信息

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

监管信息

动植物来源培养基
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16
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