rmp蛋白A琼脂糖凝胶FF，rmp Protein A Sepharose Fast Flow

图片 rmp蛋白A琼脂糖凝胶FF，rmp Protein A Sepharose Fast Flow
rmp Protein A Sepharose Fast Flow是一种低泄漏、基于非哺乳动物的亲和树脂，设计用于实验室和工艺规模的单克隆和多克隆抗体的高纯度分离。17513801；17513802；17513803；17513805；17-5138-01；17-5138-02；17-5138-03；17-5138-05
制造商品牌: (安)法玛西亚 Amersham Pharmacia , 思拓凡 Cytiva
货号（SKU）: 17513803
签订合同 √ 正规发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √

rmp Protein A Sepharose Fast Flow is a low leakage, non-mammalian based affinity resin designed for high purity separation of monoclonal and polyclonal antibodies at the laboratory and process scales.

通过化学稳定的酰胺键多点连接 rProtein A
使蛋白质 A 配体泄漏最小化的偶联化学。
rmp Sepharose 4 Sepharose Fast Flow 由重组蛋白 A 组成，通过还原胺化作用与完善的 Sepharose 4 Fast Flow 基础基质偶联，形成多点连接。

还原胺化产生化学稳定的酰胺键并导致配体泄漏率低。 rmp Protein A Sepharose 4 Fast Flow 的生产不使用动物源性成分。

作为 BioProcess 树脂系列的一员，rmp Sepharose 4 Fast Flow 能够满足工业需求，提供安全的供应以及全面的技术和法规支持。

  • Multi-point attachment of rProtein A by chemically-stable amide bonds
  • Coupling chemistry that minimizes leakage of protein A ligand.

rmp Sepharose 4 Sepharose Fast Flow is composed of recombinant Protein A coupled to the well established Sepharose 4 Fast Flow base matrix by reductive amination resulting in multipoint attachment.

Reductive amination creates chemically stable amide bonds and resulting low ligand leakage. rmp Protein A Sepharose 4 Fast Flow is manufactured without using animal-derived components.

As member of the BioProcess resins range, rmp Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

 

Parameterrmp Protein A Sepharose Fast Flow
Chromatography techniqueAntibody affinity chromatography 
BioProcess resinYes 
LigandRecombinant protein A (E.coli) 
Particle size, d50V1~90 µm 
Matrixcross-linked agarose, 4%, spherical 
Ligand Coupling MethodReductive Amination 
Binding Capacity/ml Chromatography Medium：>Min. 22 mg human IgG/mL 
pH stability, operational23–10 
pH stability, CIP33–12 
Pressure/flow characteristics4150–250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water). 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityStable in aqueous buffers commonly used in Protain A chromatography, 20% ethanol 20 mM, Sodium phosphate, 0.1 M Glycin/HCl pH 3.5, 0.1 M Glycin/HCl pH 2.5 
Certificate of AnalysisYes 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function. pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at pH below 2.”
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function. pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at pH below 2.
  • 4The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure/flow test performed on the base matrix.
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20
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