Q Sepharose XL is a strong anion exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
Q Sepharose XL 基于高度交联的、由珠子形成的 6% 琼脂糖基质，类似于成熟的 Q Sepharose Fast Flow 树脂。葡聚糖链与琼脂糖基质共价偶联，并通过化学稳定的醚键用季铵 (Q) 强阴离子交换基团进行修饰。稳定的琼脂糖基质和带有结合带电基团的葡聚糖的长而灵活的链共同作用以增加负载能力，同时允许高流速。
Q Sepharose XL 具有高负载能力和优异的物理和化学稳定性，适用于下游过程的捕获阶段，其特点是大量的粗样品负载。
作为 BioProcess 树脂系列的一员，Q Sepharose XL 凭借供应安全性以及全面的技术和法规支持满足工业需求。
- High loading capacities give more productive capture from clarified feedstocks.
- Strong anion exchanger designed for capture steps
- High chemical stability for effective CIP/sanitization
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow resin. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.
With its high loading capacities and excellent physical and chemical stability, Q Sepharose XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.
As member of the BioProcess resins range, Q Sepharose XL meets industrial demands with security of supply and comprehensive technical and regulatory support.
|Parameter||Q Sepharose XL, 300 mL|
|BioProcess resin||Yes |
|Ligand||Quaternary amine |
|Particle size, d50V1||~90 μm |
|Ion Exchanger Type||Strong anion exchanger |
|Matrix||6% cross-linked agarose with dextran surface extenders |
|Particle Size||45 μm-165 μm |
|Ligand Coupling Method||Dextran extenders through ether bonds |
|Ionic Capacity||0.18-0.26 mmol Cl-/ml medium |
|pH stability, operational2||2–12 |
|pH stability, CIP3||2–14 |
|Pressure/Flow Specification||300-500 cm/h |
|Storage||4 to 30°C, 20% Ethanol |
|Chemical stability||Commonly used buffers, nonionic detergents, 1 M NaOH, 6 M guanidine hydrochloride |
|Certificate of Analysis||Yes |
|Licensing||For licensing information, see the Licensing Statements page in the About Us section. |
|Pack size||300 mL |