MES是Good's缓冲液之一，是使用最广泛的生物缓冲液。 MES是具有吗啉环的两性离子N-取代的氨基磺酸。 它不会与环境和生物学研究中使用的大多数金属形成络合物。 它易溶于水，脂质溶解度最小，使其不透膜。 可以通过用NaOH滴定至所需的pH值来制备使用MES的缓冲液。 或者，可以将MES和MES钠盐的储备溶液混合以达到所需的pH。 使用储备溶液制备给定pH的缓冲液的标准混合表已经发布。 不建议在pH 7.4的缓冲液中使用MES。 应该考虑其他缓冲区。
MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.
MES Hydrate has been used:
• As a component of extraction buffer in the extraction of the enzyme BACE1
• As a component of the reaction mixture for the measurement of 5,10-methenyltetrahydrofolate synthetase (MTHFS) enzyme activity in fibroblasts
• As a component of MES coupling buffer during the coupling of mAb or GST-fusion proteins to carboxylate-modified polystyrene latex (CML) beads
50, 250 g in poly bottle
A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.
Storage and Stability
Solutions are stable at 2-8 C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.
Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. Similar product MES hydrate M8250 is tested at 20g/80mL water, or 1.3M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65M.
便于将 MES 产品的规格与 MES 规格表进行比较。
|impurities||Insoluble matter, passes filter test|
|ign. residue (900°C)||≤0.05%|
|pH||2.5-4.0 (0.5 M in H2O)|
|useful pH range||5.5 - 6.7|
|solubility||H2O: 0.5 M, clear, colorless|
|anion traces||chloride (Cl-): ≤0.005%|
|cation traces||Al: ≤0.0005%|
|absorption||≤0.020 at 280 in H2O at 0.5 M|
|≤0.025 at 260 in H2O at 0.5 M|
|storage temp.||room temp|
RIDADR NONH for all modes of transport