MabSelect抗体纯化层析树脂,MabSelect antibody purification chromatography resin

MabSelect 是一种 BioProcess 蛋白 A 树脂,用于从大量样品中捕获单克隆抗体 (mAb)。17519903;17519902;17519901;17519906;17519904
货号(SKU): 17519903
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₹ 1,13,041.72

MabSelect is a BioProcess protein A resin for capturing monoclonal antibodies (mAbs) from large sample volumes.

MabSelect 是一种 BioProcess 蛋白 A 树脂,用于从大量样品中捕获单克隆抗体 (mAb)。

高流速琼脂糖基质允许在生产规模上实现高流速,从而能够在一个工作日内处理超过 10 000 L 的饲料。
BioProcess 树脂支持工业应用,并在批准的工艺中得到很好的证实。
Resin 满足工业对供应安全性、强大性能和监管支持的需求。
该树脂基于具有重组蛋白 A 配体的高度交联的琼脂糖基质。该配体被设计成有利于定向偶联,从而产生具有增强的 IgG 结合能力的亲和树脂。基础基质的亲水性确保非特异性结合水平较低,从而导致洗脱池中宿主细胞来源的杂质水平较低。 MabSelect 针对高容量、高通量进行了优化,并提供了高纯度和高产量的产品库。

重组蛋白 A 配体在大肠杆菌中表达,不含哺乳动物来源的成分。与 IgG 的 Fc 区结合的特异性类似于天然蛋白 A 的特异性,可在一步中提供高纯化因子。此外,高容量和新颖的基础基质使 MabSelect 成为以工艺规模纯化 mAb 的绝佳选择。

  • High-flow agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
  • Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity, which reduces resin volume requirements.
  • Low non-specific binding resulting in low levels of impurities in the product eluate pool
  • BioProcess resin supported for industrial applications and well-established in approved processes.
  • Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.

Optimized for high capacity and throughput

The resin is based on a highly cross-linked agarose matrix with a recombinant protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity resin with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. MabSelect is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.

High purification factor in a single step

The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similarto that of native protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect an excellent choice for purification of mAbs at process scale.

Security and support

As a member of the BioProcess resins range, MabSelect meets industrial demands with security of supply and comprehensive technical and regulatory support.

Prepacked columns and other formats

MabSelect is available in the following formats: 

Process development scale – prepacked

  • PreDictor MabSelect filter plates – 96-well plates for high-throughput screening or process development.
  • PredDictor RoboColumn MabSelect– minitiaturized column format for high throughput process development
  • HiScreen MabSelect columns – optimized for method and process development .

Lab/research scale – prepacked for manual purification

HiTrap MabSelect columns – for easy use with a syringe, peristaltic pump, or chromatography system.

Lab/research scale – prepacked for automated purification and analysis

HiTrap MabSelect columns – for easy use with a syringe, peristaltic pump, or chromatography system.

Process scale purification – prepacked

ReadyToProcess MabSelect NS columns – 1 L and larger columns for clinical manufacturing that avoid the need for cleaning and cleaning validation.

Learn about prepacked HiScreen MabSelectHiTrap MabSelectReadyToProcess MabSelect columnsPreDictor MabSelect or PreDictor Robocolumns MabSelect


ParameterMabSelect, 1 L
Chromatography techniqueAntibody affinity chromatography 
BioProcess resinYes 
LigandRecombinant protein A (E.coli) 
Particle size, d50V1~85 µm 
MatrixHighly cross-linked agarose, spherical 
Ligand Coupling MethodEpoxy activation 
Dynamic binding capacity, QB102~30 mg hlgG/mL resin 
pH stability, operational33–10 
pH stability, CIP43–12 
Storage2 to 8°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol) 
Chemical stabilityStable in aqueous buffers commonly used in Protein A Chromatography; 10 mM NaOH (pH 12), 0.1 M 20% sodium citrate/HCl (pH 3), 6M Gua-HCl, 8M urea, 20% ethanol, 2% benzyl alcohol 
Certificate of AnalysisYes 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2Dynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a XK 16/20 at 20 cm bed height (2.4 min residence time) for human IgG in 0.020 M NaH₂PO₄, pH 7.4
  • 3pH range where resin can be operated without significant change in function .
  • 4pH range where resin can be subjected to cleaning-in-place without significant change in function pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at pH below 2. Reducing agent e.g. 100 mM 1-Thioglycerol followed by 15 mM NaOH is among the most efficient CIP for MabSelect.
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16