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NHS-LC-双吖丙啶)(琥珀酰亚胺基6-(4,4′-氮杂戊酰胺基)己酸酯,NHS-LC-Diazirine) (succinimidyl 6-(4,4′-azipentanamido)hexanoate [LC-SDA];≥90%

Empirical Formula (Hill Notation): C15H22N4O5 分子量: 338.36 PubChem化学物质编号: 329768806 NACRES: NA.22
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): 803421
签订合同 √ 正规发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √
¥5,861.45

说明

一般描述

Succinimidyl-diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions, and these SDA reagents extend the efficiency and range of interactions that can be explored by this approach.
 

特点和优势

  • Membrane-permeable—suitable for in vivo intracellular protein crosslinking
  • Heterobifunctional—NHS ester group reacts with primary amines at pH 7 to 9 to form covalent amide bonds; diazirine (azipentanoate) group reacts efficiently with any amino acid side chain or peptide backbone upon activation with long-wave UV light (330-370 nm)
  • Controllable—two-step chemical crosslinking is activated using common laboratory UV lamps
  • Easy to use—these crosslinkers are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark
  • Better than aryl azides—the diazirine photoreactive group has better photostability in normal light than phenyl azide groups of traditional photoreactive crosslinkers, yet the diazirine group is more efficiently activated by long-wave UV light

注意

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.
 
 

属性

检测方案

≥90%

质量水平

形式

powder

分子量

338.36

reaction suitability

reagent type: cross-linking reagent

储存条件

desiccated

溶解性

DMSO or DMF: soluble

运输

ambient

储存温度

2-8°C

SMILES string

CC1(N=N1)CCC(NCCCCCC(ON2C(CCC2=O)=O)=O)=O

InChI

1S/C15H22N4O5/c1-15(17-18-15)9-8-11(20)16-10-4-2-3-5-14(23)24-19-12(21)6-7-13(19)22/h2-10H2,1H3,(H,16,20)

InChI key

DYNYVLFTFJHMCW-UHFFFAOYSA-N

 

安全信息

储存分类代码

11 - Combustible Solids

WGK

WGK 3

商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20