LB培养基 [LB肉汤],LB Broth (Lennox);Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

别名:Lennox LB;Luria Bertani肉汤;伦诺克斯肉汤;Lennox broth;Luria Bertani Broth;NACRES NA.85
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): L3522
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General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.


LB Broth (Miller) has been used as a culture medium for E coli,[3] Pseudomonas putida KT2440,[4] Vibrio parahaemolyticus,[5] and soil samples for the isolation of Bacillus spp.[6]
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Biochem/physiol Actions

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component[2].

Features and Benefits

Miller LB粉末:
• 包装尺寸更大,易于扩大规模
• 液体的廉价替代品
• 标准配方

Preparation Note

1. Suspend 25 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.


搅拌,使25g粉末悬浮于1L水中。 在121℃高压灭菌15分钟。 先冷却再添加其他成分,如抗生素(如果需要)。


Quality Level200
gradefor molecular biology
descriptionMiller′s Modification, 10 g/L NaCl
compositionNaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
pH range6.8 - 7.2 (2.5% solution)
Featured IndustryFood and Beverages
storage temp
suitabilitynonselective for Escherichia coli
nonselective for coliforms


个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 3
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32