L-谷氨酰胺，L-Glutamine；EMPROVE® ESSENTIAL DAB,USP, 99.0-101.0 %

EMPROVE^® ESSENTIAL DAB,USP

 

L-谷氨酰胺是一种必需氨基酸，作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉及冷冻溶液的状态下极为稳定。然而，L-谷氨酰胺在液体培养基或储存溶液中均会相对较快的降解。最佳的细胞表现通常需要在使用前向培养基中添加L-谷氨酰胺补充剂。

 

Assay (perchloric acid titration, calculated on dried substance)99.0 - 101.0 %Identity (IR-spectrum)passes testAppearancewhite to almost white, fine powderAppearance of solution (25 g/l; CO₂-free water)clear and colorlessSpec. rotation (α 20/D, 100 g/l, hydrochloric acid 2 mol/l, calc. on dried substance)+31.5 to +33.0Spec. rotation (α 20/D, 40 g/l, water, calc. on dried substance)+6.3 to +7.3pH (25 g/l; CO₂-free water)4.0 - 6.0Chloride (Cl)≤ 200 ppmSulfate (SO₄)≤ 200 ppmHeavy metals (as Pb)≤ 10 ppmCu (Copper)≤ 25 ppmFe (Iron)≤ 10 ppmFe (Iron) (ICP)≤ 10 ppmMn (Manganese)≤ 25 ppmNi (Nickel)≤ 2 ppmZn (Zinc)≤ 130 ppmNH₄ (Ammonium)≤ 0.1 %Ninhydrin - positive substances (TLC)≤ 0.5 %Residual solvents (ICH Q3C)excluded by manufacturing processSulfated ash (600 °C)≤ 0.1 %Loss on drying (105 °C; 3 h)≤ 0.3 %Bacterial endotoxins≤ 2.0 I.U./gElemental impurity specifications have been set considering ICH Q3D (Guideline for Elemental Impurities). Class 1-3 elements are not likely to be present above the ICH Q3D option 1 limit, unless specified and indicated (*).Corresponds to DAB, USP

 

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany