Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.
|Quality Level ||200|
|biological source ||human|
|recombinant ||expressed in baculovirus infected Sf21 cells|
|assay ||≥97% (SDS-PAGE and N-terminal analysis)|
|form ||lyophilized powder|
|potency ||0.01-0.05 ng/mL ED50|
|mol wt ||glycoprotein 75 kDa|
|packaging ||pkg of 5 μg|
|application(s) ||cell culture | mammalian: suitable|
|impurities ||endotoxin, tested|
|UniProt accession no. ||P29459,|
|storage temp. ||−20°C|
|Gene Information ||human ... IL12A(3592), IL12B(3593)|
RIDADR NONH for all modes of transport