Interleukin-10 (IL-10) human has been used to study the effect of IL-10 on cartilage proteoglycan turnover. It has been used as an antigen to analyse the reactivity patterns of autoantibodies in healthy humans and humans suffering from type 1 diabetes mellitus.
IL-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.
Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, containing 50 μg bovine serum albumin/μg IL-10
|Quality Level ||200|
|biological source ||human|
|recombinant ||expressed in baculovirus infected Sf21 cells|
|assay ||≥97% (SDS-PAGE)|
|form ||lyophilized powder|
|potency ||0.1-0.5 ng/mL EC50|
|mol wt ||18 kDa|
|packaging ||pkg of 5 μg|
|application(s) ||cell culture | mammalian: suitable|
|impurities ||endotoxin, tested|
|UniProt accession no. ||P22301|
|storage temp. ||−20°C|
|Gene Information ||human ... IL10(3586)|
RIDADR NONH for all modes of transport