IL-9 is an immunoregulatory cytokine produced by IL-2 activated Th2 lymphocytes. IL-9 enhances the proliferation of T lymphocytes, mast cells, erthroid precursor cells and megakaryoblastic leukemia cell lines. Over-expression of IL-9 has been implicated in the pathogenesis of anaplastic lymphoma and Hodgkin’s disease. Whereas murine IL-9 can function on human cells, human IL-9 is inactive on mouse cells. Recombinant human IL-9 is a 14.0 kDa protein of 127 amino acid residues including 10 cysteine residues which are fully conserved between the human murine proteins.
IL-9 is an immunoregulatory cytokine produced by IL-2 activated Th2 lymphocytes. Recombinant human IL-9 is a 14.0 kDa protein of 127 amino acid residues including 10 cysteine residues which are fully conserved between the human murine proteins.
MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
|biological source ||human|
|recombinant ||expressed in E. coli|
|assay ||≥98% (HPLC)|
| ||≥98% (SDS-PAGE)|
|potency ||≤1 ng/mL ED50|
|mol wt ||14.0 kDa|
|packaging ||pkg of 10 μg|
|application(s) ||cell culture | mammalian: suitable|
|impurities ||<0.1 EU/μg endotoxin, tested|
|color ||white to off-white|
|UniProt accession no. ||P15248|
|shipped in ||wet ice|
|storage temp. ||−20°C|
|Gene Information ||human ... IL9(3578)|
RIDADR NONH for all modes of transport