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IL-12 human,[人IL-12, IL12, TCMF, CLMF, NKSF, TSF];recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

人白介素12;人白细胞介素12;CTL maturation factor (TCMF),, Cytotoxic lymphocyte maturation factor (CLMF), NKSF, TSF
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRP3073
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¥3,574.00

产品描述

General description

IL-12 (interleukin 12) is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Recombinant Human IL-12 is a 75kDa heterodimeric glycoprotein consisting of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits (503 total amino acid residues).

Biochem/physiol Actions

IL-12 (interleukin 12) is a proinflammatory cytokine and plays an important role in protection against pathogens.[4] IL-12 is a potent regulator of cell mediated immune responses and it induces IFN (interferon)-γ production by NK (natural killer) and T cells.[1][2] Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 (T helper) cells. Polymorphisms in the IL-12 gene are associated with risk of rheumatoid arthritis.[3] It also exhibits anti-tumor activities by enhancing Th1 adaptive immunity and cytotoxic T lymphocyte functions.[5]

Sequence

p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Physical form

Lyophilized from 0.5x PBS, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2-7.4 to a concentration of 0.2 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

性质

biological source  human
recombinant  expressed in CHO cells
assay  ≥98% (HPLC)
 ≥98% (SDS-PAGE)
form  lyophilized
potency  ≤1.0 ng/mL ED50
mol wt  40 kDa
packaging  pkg of 10 μg
application(s)  cell culture | mammalian: suitable
impurities  <0.1 EU/μg endotoxin, tested
color  white to off-white
UniProt accession no.  P29459
 P29460
shipped in  wet ice
storage temp.  −20°C
Gene Information  human ... IL12A(3592)


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16