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IL-10 human,[人IL-10, IL10, B-TCGF, CSIF, TGIF];Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Interleukin-10 human;Recombin human IL10 protein;CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF;NACRES NA.32
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRP3312
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¥4,106.86

产品描述

General description

Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes.[1] Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. The gene encoding this protein is localized on human chromosome 1q31-32.[2] Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues. Interleukin 10 (IL-10) comprises interferon IFN-γ fold and exists as a symmetric homodimer. It shares structural similarity with interferon γ (IFN-γ).

Application

Interleukin 10 (IL-10) human has been used in enzyme-linked immunosorbent assay.[10]

Biochem/physiol Actions

Interleukin-10 (IL-10) is an anti-inflammatory protein that inhibits the expression of proinflammatory cytokines such as IL-1 and tumor necrosis factor-α (TNF-α). Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. It inhibits the production of IFN-γ and antigen-specific proliferation of type-1 T helper cells (Th1). It protects the host tissue from damage during immune responses. The protein has a role in modulating viral immunity. IL-10 enhances cytokine production, natural killer cell proliferation and cytotoxicity in vitro.[1][3] IL-10 is an immunosuppressive cytokine.[8] It promotes wound repair and functions to resolve inflammation in the central nervous system.[8] IL-10 mediates its signaling function through the interleukin receptors namely, IL-10RA and two IL-10RBs.[6] The expression of IL-10 is regulated by signal transducer and activator of transcription 3 (STAT3) and suppressor of cytokine signaling-3 (SOCS3) ratios. Polymorphisms in the IL-10 gene is implicated in breast cancer.[6]

Physical form

Lyophilized from 20 mM Sodium Phosphate, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

性质

biological source  human
recombinant  expressed in E. coli
assay  ≥98% (HPLC)
 ≥98% (SDS-PAGE)
form  lyophilized
potency  ≤2.0 ng/mL
mol wt  18.6 kDa
packaging  pkg of 10 μg
impurities  endotoxin, tested
UniProt accession no.  P22301
shipped in  wet ice
storage temp.  −20°C
Gene Information  human ... IL10(3586)


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 60 | 欧洲Eur. ≥ 21 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 10