IgG琼脂糖凝胶6FF亲和树脂,IgG Sepharose 6 Fast Flow affinity resin

IgG Sepharose 6 Fast Flow 使用与人 IgG 共价偶联的刚性 Sepharose 6 Fast Flow 基质来纯化含蛋白质 A 的蛋白质。17096902;17096901
货号(SKU): 17096902
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lgG Sepharose 6 Fast Flow uses the rigid Sepharose 6 Fast Flow matrix, covalently coupled with human IgG, to purify protein A-containing proteins.

专为蛋白质 A 和蛋白质 A 融合偶联物的快速、单步纯化而设计。
结合至少 2 mg 蛋白 A/mL 树脂。
适用于蛋白质复合物的串联亲和纯化 (TAP)。
使用 IgG Sepharose 6 Fast Flow 的应用包括:

串联亲和纯化 (TAP) 从人体细胞中获取功能蛋白
串联亲和纯化以研究 RNA 聚合酶 II Rpb1 C 端结构域上的磷酸化位点
分离蛋白 A-Cvt19 以研究细胞质到液泡靶向 (Cvt) 途径

  • Designed for rapid, single-step purification of protein A and protein A fusion conjugates.
  • Binds at least 2 mg protein A/mL resin.
  • Suitable for tandem affinity purification (TAP) of protein complexes.

Successful use in published research

Applications using IgG Sepharose 6 Fast Flow include:

Compatible empty columns

Cytiva offers a wide range of Life Sciences solutions for protein purification, including several types of empty columns. lgG Sepharose 6 Fast Flow is compatible with gravity flow, Tricorn, HiScale, and XK column lines.

Convenient prepacked columns

IgG Sepharose Fast Flow resin is available in the following format:

  • HiTrap IgG Sepharose FF: convenient for easy use with a syringe, peristaltic pump, or chromatography system


ParameterIgG Sepharose 6 Fast Flow, 200 mL
Order InformationThis product is produced based on a customer order. Delivery time may be longer than for standard products. 
LigandHuman IgG 
Particle size, d50V1~90 µm 
MatrixHighly cross-linked 6% agarose 
Binding Capacity/ml Chromatography Medium~3 mg Protein A 
ApplicationRecombinant tagged proteins containing a protein A tag 
pH stability, operational23–10 
pH stability, CIP33–10 
Pressure/Flow SpecificationBase matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm 
Flow velocity< 300 cm/h, using XK 50/30 column with 15 cm bed height 
Storage2 to 8ºC, 20% Ethanol, 50 mM K₂HPO₄, 1mM MgCl₂ pH 7.2 in 20% Ethanol 
Chemical stabilityStable to commonly used aqueous buffers 
Certificate of AnalysisYes 
Pack size200 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32