HVEM/TNFRSF14 human，[ATAR, HVEA, LIGHTR, TR2]；recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

General description

Herpesvirus entry mediator (HVEM), also known as TNFRSF14, TR2 (TNF receptor like molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

性质

biological source	human
recombinant	expressed in HEK 293 cells
assay	≥95% (SDS-PAGE)
form	lyophilized
mol wt	monomer observed mol wt 50-60 kDa by SDS-PAGE (reducing) (DTT-reduced.)
	calculated mol wt 45.4 kDa (This protein is a disulfide-linked homodimeric protein after removal of the signal peptide. The 413 aa reduced monomer is fused at the C-terminus to human IgG1 (rhHVEM-Fc).)
packaging	pkg of 20 and 100 μg
impurities	<1 EU/μg endotoxin (LAL test)
UniProt accession no.	Q92956
shipped in	wet ice
storage temp.	−20°C
Gene Information	human ... TNFRSF14(8764)

安全信息