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Granulocyte Colony-Stimulating Factor from mouse;G-CSF, recombinant, expressed in E. coli, suitable for cell culture

Recombinant Mouse Granulocyte Colony-Stimulating Factor/G-CSF/CSF1(C-polyHis);MDL number MFCD00166475 NACRES NA.77
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): G8160
MDL号: MFCD00166475
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¥3,431.05

产品描述

General description

The Csf3r (colony stimulating factor 3 receptor (granulocyte)) gene encodes a prototypical mobilizing cytokine.[3]

Application

Granulocyte Colony-Stimulating Factor from mouse has been used in cytokine therapy.[1]

Biochem/physiol Actions

G-CSF is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Granulocyte colony-stimulating factor (G-CSF) is a lineage-restricted hematopoietic growth factor, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors. G-CSF3,4 is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and mature granulocytes, and promotes differentiation of certain myeloid leukemic cells. Natural G-CSF is a glycoprotein of 177 amino acids and a molecular weight of 19 kDa.5 Human and murine G-CSF have about 75% homology and show biological cross-reactivity.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM citric acid, 0.01% TWEEN® 20, pH 4.0, containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

法律信息

TWEEN is a registered trademark of Croda International PLC

性质

Quality Level  100
biological source  mouse
recombinant  expressed in E. coli
assay  >97% (SDS-PAGE)
form  lyophilized powder
potency  10-60 pg/mL ED50
quality  endotoxin tested
mol wt  19 kDa (179 amino acids including N-terminal methionine)
packaging  pkg of 5 and 25 μg
storage condition  avoid repeated freeze/thaw cycles
application(s)  cell culture | mammalian: suitable
impurities  ≤0.01 EU/μg
UniProt accession no.  P09920
storage temp.  −20°C
Gene Information  mouse ... Csf3(12985), Csf3r(12986)


安全信息

符号 
GHS07  GHS07
信号词 Warning
危害声明 H319
个人防护装备 EyeshieldsGlovestype N95 (US)
危害码 (欧洲) Xi
风险声明 (欧洲) 36
安全声明 (欧洲) 26
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 60 | 欧洲Eur. ≥ 21 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 10