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蛋白G亲和琼脂糖凝胶抗体纯化树脂,GammaBind Plus Sepharose antibody purification resin

GammaBind Plus Sepharose 是一种蛋白G亲和层析树脂,用于纯化多种物种的免疫球蛋白 (IgG)。17088604;17088602;17088601
货号(SKU): 17088604
增值税发票 √ 免费送货 √ 订货电话:010-68689484
€921.36

GammaBind Plus Sepharose is a protein G affinity chromatography resin for purifying immunoglobulins (IgGs) from a variety of species.

GammaBind Plus Sepharose 是一种蛋白 G 亲和层析树脂,用于纯化多种物种的免疫球蛋白 (IgG)。

与 GammaBind G Sepharose 相比,与大鼠和小鼠 IgG 亚类的结合增强。
与所有小鼠、大鼠和人 IgG 亚类结合;结合豚鼠、兔、山羊、牛、绵羊和马的总 IgG。
实验室规模的 IgG 纯化产量高达 35 mg 人 IgG/mL。
非常适合免疫沉淀程序。
用于 IgG 纯化的亲和层析树脂
GammaBind Plus Sepharose 用于分析和纯化任何生物流体或细胞培养基中的免疫球蛋白类别、亚类和片段。这种树脂也适用于免疫沉淀。

这种亲和层析树脂是 GammaBind G,3 型,通过马来酰亚胺键共价固定在 Sepharose CL-6B 上。这种重组形式的链球菌 G 蛋白与来自多种哺乳动物的 IgG 的 Fc 区强烈结合。此外,许多抗体还通过 Fab 区与 Protein G 上的低亲和力位点相互作用。

  • Enhanced binding to rat and mouse IgG subclasses compared with GammaBind G Sepharose.
  • Binds to all mouse, rat, and human IgG subclasses; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  • Lab-scale IgG purification yields up to 35 mg human IgG/mL.
  • Well suited for immunoprecipitation procedures.

Affinity chromatography resin for IgG purification

GammaBind Plus Sepharose is used to analyze and purify classes, subclasses, and fragments of immunoglobulins from any biological fluid or cell culture medium. This resin is also suitable for immunoprecipitation.

This affinity chromatography resin is GammaBind G, Type 3, covalently immobilized to Sepharose CL-6B by maleimide linkage. This recombinant form of streptococcal protein G binds strongly to the Fc region of IgG from a variety of mammalian species. In addition, many antibodies also interact via the Fab region with a low affinity site on Protein G.

Successful use in published research

GammaBind Plus Sepharose is used in a variety of applications, including:

Compatible empty columns

Cytiva solutions include a variety of empty columns for packing. GammaBind Plus Sepharose resin is compatible for use with Tricorn, XK and HiScale columns.

Other antibody purification resins from Cytiva

Looking for a different product? Browse Cytiva's full antibody affinity chromatography range. Or let Cytiva help you find the right antibody purification product for your application.

 

ParameterGammaBind Plus Sepharose, 25 mL
Chromatography techniqueAntibody affinity chromatography 
LigandRecombinant GammaBind G type 3 lacking albumin-binding region 
Particle size, d50V1~90 µm 
MatrixCross-linked agarose, 6% 
Ligand Coupling MethodMaleimide activation 
Ligand density3 mg GammaBind G, type 3/ml medium 
pH stability, operational23–9 
pH stability, CIP32–9 
Flow velocity< 130 cm/h 
Storage2 to 8°C, 20% Ethanol 
Chemical stabilityCommonly used aqueous buffers 
Pack size25 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16