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Capto蓝色亲和层析树脂 [高取代],Capto Blue (high sub) affinity chromatography resin

一种用于捕获人血清白蛋白的亲和树脂。 也用于纯化 HSA 融合蛋白、凝血因子、酶和重组蛋白。17545201;17545205;17545204;17545202
货号(SKU): 17545202
增值税发票 √ 免费送货 √ 订货电话:010-68689484
$655.78

An affinity resin for capturing human serum albumin. Also for purification of HSA fusion proteins, blood coagulation factors, enzymes, and recombinant proteins.

出色的化学稳定性可确​​保耐受重复原位清洁和消毒程序中使用的刺激性溶剂。
高刚性琼脂糖基质允许高流速和大体积样品的处理。
Cibacron Blue 配体功能可以通过使用适当的缓冲盐和缓冲液电导率来修改,以增加对所需目标的选择性。
在实验室和工艺规模上去除或纯化蛋白质的绝佳选择。
Resin 满足工业对供应安全性、强大性能和监管支持的需求。
定制设计的树脂
Capto Blue(高低音)通过定制设计媒体提供,并且仅根据客户需求生产。因此,交货时间通常比标准树脂(例如 Capto Blue)要长。

与 Capto Blue 树脂的比较
Capto Blue (high sub) 的应用与标准 Capto Blue 树脂的应用相同。但配体浓度更高:~ 18 μmol Cibacron Blue/mL Capto Blue(高亚型)树脂。 ~ 13 μmol Cibacron Blue/mL Capto Blue 树脂。

  • Excellent chemical stability ensures tolerance of harsh solvents used in repeated cleaning-in-place and sanitization procedures.
  • Highly rigid agarose base matrix allows high flow rates and processing of large sample volumes.
  • Cibacron Blue ligand functionality may be modified through the use of appropriate buffer salts and buffer conductivity to increase selectivity for desired targets.
  • Excellent choice for the removal or purification of proteins at both laboratory and process scales.
  • Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.

A custom designed resin

Capto Blue (high sub) is made available through Custom Design Media and is only produced on customer demand. Therefore delivery time is typically longer than for standard resin, such as Capto Blue.

Comparison with Capto Blue resin

The applications of Capto Blue (high sub) are the same as for the standard Capto Blue resin. But the ligand concentration is higher: ~ 18 μmol Cibacron Blue/mL Capto Blue (high sub) resin. ~ 13 μmol Cibacron Blue/mL Capto Blue resin.

 

ParameterCapto Blue (High Sub), 500 mL
Chromatography techniqueGroup specific affinity 
BioProcess resinYes 
LigandCibacron blue 
Particle size, d50V1~75 µm 
MatrixHighly cross-linked agarose 
Ligand Coupling MethodEther linkages and hydrophilic spacer arm 
Binding Capacity/ml Chromatography Medium> 30 mg HSA/mL resin (Qb10 at 4 min residence time) 
Ligand densityApprox. 18 μmol/ml 
pH stability, operational23–13 
pH stability, CIP32–13.5 
Pressure/Flow Specification300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height 
Coupling ChemistryAmine functional spacer 
Storage4 to 8°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol 
Chemical stabilityStable to commonly used aqueous buffers:0,5 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 
Autoclavable120°C at 2.6 bar 
Pack size500 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16