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APN-叠氮化物,APN-Azide [APN-N3];95%

别名:3-(4-叠氮基苯基)丙炔腈;3-(4-Azidophenyl)propiolonitrile;APN-N3; Empirical Formula (Hill Notation): C9H4N4 CAS号: 1643841-88-6 分子量: 168.15 PubChem化学物质编号: 329769021
制造商品牌: Sigma-Aldrich
货号(SKU): 806595
CAS号: 1643841-88-6
技术支持 √ 增值税发票 √ 免费送货 √ 质量保障 √
¥913.13

说明

应用

APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.

Standard protein labeling procedure (cysteine labeling)
1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
3. Incubate at room temperature for 2 hours.
4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.
5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.
*Note: avoid thiol-containing buffers.
 
 

属性

质量水平

检测方案

95%

形式

powder

reaction suitability

reagent type: cross-linking reagent

mp

100 °C

官能团

azide

储存温度

2-8°C

SMILES string

N#CC#CC1=CC=C(N=[N+]=[N-])C=C1

InChI

1S/C9H4N4/c10-7-1-2-8-3-5-9(6-4-8)12-13-11/h3-6H

InChI key

BDLXITYEYYHQNK-UHFFFAOYSA-N

 

安全信息

象形图

FlameExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

4.1B - Flammable solid hazardous materials

WGK

WGK 3

商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 55 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 8 | 香港与北京 ≥ 10