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ANX琼脂糖凝胶4FF [高取代]，ANX Sepharose 4 Fast Flow (High Sub)

图片 ANX琼脂糖凝胶4FF [高取代]，ANX Sepharose 4 Fast Flow (High Sub)
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ANX Sepharose 4 Fast Flow (high sub)是一种弱阴离子交换剂，与传统离子交换剂相比具有不同的选择性。 它针对较大蛋白质的分离进行了优化。17128760；17128710；17128704；17128705；17-1287-60；17-1287-10；17-1287-04；17-1287-05
制造商品牌: (安)法玛西亚 Amersham Pharmacia , 思拓凡 Cytiva
货号（SKU）: 17128701
增值税发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √
¥3,360.00

ANX Sepharose 4 Fast Flow (High Sub)

ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins。

ANX Sepharose 4 Fast Flow (high sub)是一种弱阴离子交换剂，与传统离子交换剂相比具有不同的选择性。 它针对较大蛋白质的分离进行了优化。

  • Weak anion exchanger with different selectivity compared with traditional ion exchangers.
  • Applicable for separation of high molecular weight proteins.
  • Developed in co-operation with leading large-scale pharmaceutical manufacturers.
  • High chemical stability allows for well proven CIP and sanitization protocols.
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

ANX Sepharose Fast Flow (high sub) is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger resin with tertiary amine groups attached to the base matrix Sepharose 4 Fast Flow via ether linkage and a hydrophilic spacer arm. The diethylaminopropylgroup is coupled in a mode that precludes formation of quaternary groups, which are typically seen on traditional DEAE media, resulting in a truly weak anion exchanger. The base matrix, Sepharose 4 Fast Flow, is a highly cross-linked, 4% agarose derivative with high chemical and physical stability and broad separation range. ANX Sepharose 4 Fast Flow (high sub) has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.

As member of the BioProcess resin range, ANX Sepharose 4 Fast Flow (high sub) meets industrial demands with security of supply and comprehensive technical and regulatory support.

 

参数ANX Sepharose 4 Fast Flow (High Sub)
BioProcess resinYes 
Chemical stability 1Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol 
Exclusion limit (Mr) (globular proteins)：~3 × 10⁷ 
Ion Exchanger TypeWeak anion exchanger 
Ionic Capacity0.13-0.18 mmol Cl-/ml medium 
LigandDiethylaminopropyl 
Ligand Coupling MethodEther linkages and hydrophilic spacer arm 
Matrixcross-linked agarose, 4%, spherical 
Particle size, d50V 2~90 µm 
Pressure/flow characteristics 3≥200 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water). 
Storage4 to 30°C, 20% Ethanol 
pH ligand fully charged 4Below 9 
pH stability, CIP 52–14 
pH stability, operational 63–13 
  • 11 M NaOH and acetic acid should be used only for cleaning purposes.
  • 2Median particle size of the cumulative volume distribution.
  • 3The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure/flow test performed on the base matrix.
  • 4pH range where ligand is fully charged.
  • 5pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
  • 6pH range where resin can be operated without significant change in function.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20
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