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ANX琼脂糖凝胶4FF [高取代],ANX Sepharose 4 Fast Flow (High Sub)

ANX Sepharose 4 Fast Flow (high sub) 是一种弱阴离子交换剂,与传统离子交换剂相比具有不同的选择性。 它针对较大蛋白质的分离进行了优化。17128760;17128710;17128704;17128705
货号(SKU): 17128701
增值税发票 √ 免费送货 √ 订货电话:010-68689484
₹ 28,472.66

ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins。ANX琼脂糖凝胶4FF [高取代],是一种弱阴离子交换剂,与传统离子交换剂相比具有不同的选择性。 它针对较大蛋白质的分离进行了优化。

与传统离子交换剂相比具有不同选择性的弱阴离子交换剂。
适用于分离高分子量蛋白质。
与领先的大型制药商合作开发。
高化学稳定性允许经过充分验证的 CIP 和消毒协议。
基础基质的亲水性确保非特异性结合水平较低,从而导致洗脱池中宿主细胞来源的杂质水平较低。

ANX Sepharose Fast Flow (high sub) 是 Sepharose Fast Flow 离子交换平台的一部分,该平台在近几十年来一直是离子交换色谱的工业标准。 ANX Sepharose 4 Fast Flow (high sub) 是一种弱阴离子交换树脂,叔胺基团通过醚键和亲水间隔臂连接到基础基质 Sepharose 4 Fast Flow。二乙基氨基丙基的偶联模式可以防止形成季基,这通常在传统的 DEAE 介质中可见,从而产生真正弱的阴离子交换剂。基本基质 Sepharose 4 Fast Flow 是一种高度交联的 4% 琼脂糖衍生物,具有高化学和物理稳定性以及广泛的分离范围。 ANX Sepharose 4 Fast Flow (high sub) 具有很高的化学稳定性,可实现久经考验的就地清洗 (CIP) 和消毒方案。

作为 BioProcess 树脂系列的成员,ANX Sepharose 4 Fast Flow (high sub) 凭借供应安全性和全面的技术和法规支持满足工业需求。

 

  • Weak anion exchanger with different selectivity compared with traditional ion exchangers.
  • Applicable for separation of high molecular weight proteins.
  • Developed in co-operation with leading large-scale pharmaceutical manufacturers.
  • High chemical stability allows for well proven CIP and sanitization protocols.
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

ANX Sepharose Fast Flow (high sub) is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger resin with tertiary amine groups attached to the base matrix Sepharose 4 Fast Flow via ether linkage and a hydrophilic spacer arm. The diethylaminopropylgroup is coupled in a mode that precludes formation of quaternary groups, which are typically seen on traditional DEAE media, resulting in a truly weak anion exchanger. The base matrix, Sepharose 4 Fast Flow, is a highly cross-linked, 4% agarose derivative with high chemical and physical stability and broad separation range. ANX Sepharose 4 Fast Flow (high sub) has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.

As member of the BioProcess resin range, ANX Sepharose 4 Fast Flow (high sub) meets industrial demands with security of supply and comprehensive technical and regulatory support.

 

ParameterANX Sepharose 4 Fast Flow (High Sub), 500 mL
BioProcess resinYes 
LigandDiethylaminopropyl 
Particle size, d50V1~90 µm 
Ion Exchanger TypeWeak anion exchanger 
Matrixcross-linked agarose, 4%, spherical 
Ligand Coupling MethodEther linkages and hydrophilic spacer arm 
Ionic Capacity0.13-0.18 mmol Cl-/ml medium 
pH stability, operational23–13 
pH stability, CIP32–14 
pH ligand fully charged4Below 9 
Pressure/flow characteristics5≥200 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water). 
Exclusion limit (Mr) (globular proteins)~3 × 10⁷ 
Storage4 to 30°C, 20% Ethanol 
Chemical stability6Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol 
Pack size500 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
  • 4pH range where ligand is fully charged.
  • 5The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure/flow test performed on the base matrix.
  • 61 M NaOH and acetic acid should be used only for cleaning purposes.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16