General description
Diacylglycerol/diglyceride (DAG) is a glycerolipid, that is located at the plasma membrane.[8] It is made up of two fatty acid chains that are covalently attached to a single glycerol molecule with the help of an ester linkage.[9]Application
18:1 DG has been used as a diacyl-glycerol source in diacylglycerol O-acyltransferase 1 (DGAT1) assay.[6] It has also been used as substrate to assess compounds as potential inhibitors of DGAT-1 in DGAT-1 enzyme assay.[7] It is suitable to use in lipid-protein overlay screen assay with the recombinant protein His-AtROP6.
包装
5 mL PTFE Vial with Screw Cap (800811O-10mg)
5 mL PTFE Vial with Screw Cap (800811O-200mg)
5 mL PTFE Vial with Screw Cap (800811O-25mg)
5 mL PTFE Vial with Screw Cap (800811O-500mg)
Storage and Stability
Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.
Other Notes
Delivery to cells:
Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.
Effective concentration:
Most biological responses saturate at 20 to 250 μM sn-1,2-dioctanoylglycerol. Only sn-1,2 isomers appear to be active.
Precaution: Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.