1,2-二油酰基-sn-甘油 [18:1 DG, DOG]，1-2-dioleoyl-sn-glycerol, neat oil；>99% (TLC)

General description

Diacylglycerol/diglyceride (DAG) is a glycerolipid, that is located at the plasma membrane.^[8] It is made up of two fatty acid chains that are covalently attached to a single glycerol molecule with the help of an ester linkage.^[9]

Application

18:1 DG has been used as a diacyl-glycerol source in diacylglycerol O-acyltransferase 1 (DGAT1) assay.^[6] It has also been used as substrate to assess compounds as potential inhibitors of DGAT-1 in DGAT-1 enzyme assay.^[7] It is suitable to use in lipid-protein overlay screen assay with the recombinant protein His-AtROP6.

包装

5 mL PTFE Vial with Screw Cap (800811O-10mg)

5 mL PTFE Vial with Screw Cap (800811O-200mg)

5 mL PTFE Vial with Screw Cap (800811O-25mg)

5 mL PTFE Vial with Screw Cap (800811O-500mg)

Storage and Stability

Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.

Other Notes

Delivery to cells:
Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.

Effective concentration:
Most biological responses saturate at 20 to 250 μM sn-1,2-dioctanoylglycerol. Only sn-1,2 isomers appear to be active.

Precaution: Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.