脂多糖来源于肠沙门氏菌肠炎血清型；Lipopolysaccharides from Salmonella enterica serotype enteritidis [LPS]；purified by ion-exchange chromatography

This product is extracted from Salmonella enteritidis and purified by ion exchange. The source strain is ATCC 13076. Lipopolysaccharide from Salmonella enteritidis is mutagenic in murine spleen cells and causes a plaque-forming response in those cells.

 

脂多糖 (LPS) 是革兰氏阴性菌细胞壁的特征性成分。LPS 及其脂质 A 部分通过 Toll 样受体 4 (TLR4) 刺激天然免疫系统的细胞。

 

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

 

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.