潮霉素B，Hygromycin B；suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder

图片 潮霉素B，Hygromycin B；suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder
别名：潮霉素B抗生素；潮霉素B来源于吸水链霉菌；湿霉素乙；效高素；潮霉素 B干粉；homomycin

Empirical Formula (Hill Notation): C20H37N3O13 CAS号: 31282-04-9 分子量: 527.52 EC 号: 250-545-5 MDL编号: MFCD06795479 PubChem化学物质编号: 329815270 NACRES: NA.72
制造商品牌: 西格玛 Sigma-Aldrich
货号（SKU）: H9773
CAS号: 31282-04-9
MDL号: MFCD06795479
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说明

一般描述

Chemical structure: aminoglycoside
Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
 

应用

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Hygromycin B from Streptomyces hygroscopicus is used for gene cloning and ectopic expression. It is also used in MS medium for the in vitro screening of transgenic plants.
 

生化/生理作用

Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans. It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation. The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.
作用机制：本品通过诱导原核生物中m-RNA模板的误读，抑制蛋白质合成，具有抑制翻译的作用。
抗菌谱：潮霉素B对细菌、真菌和高等真核细胞有抑制作用。
 

注意

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.
 

制备说明

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.
 
 

属性

生物来源

Streptomyces hygroscopicus

质量水平

200

产品线

BioReagent

测定

≥60% (HPLC)

形式

lyophilized powder

纯化方式

ion-exchange chromatography

technique(s)

cell culture | plant: suitable

颜色

faintly brown to brown
white to beige

(1) 7.1, (2) 8.8

溶解性

H2O: soluble 50 mg/mL
ethanol: soluble
methanol: soluble

抗生素抗菌谱

fungi

application(s)

agriculture

作用机制

protein synthesis | interferes

储存温度

2-8°C

SMILES string

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

 

安全信息

象形图

Skull and crossbones
GHS06

警示用语：

Danger

危险声明

H301 - H312 - H330

危险分类

Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal

储存分类代码

6.1B - Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 50 | 欧洲Eur. ≥ 12 | 東京Tokyo ≥ 20 | 香港与北京 ≥ 50
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