溶壁酶来源于藤黄节杆菌，Lyticase from Arthrobacter luteus；lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

Lyticase from Arthrobacter luteus has been used to lyse the fungal cell wall for DNA isolation.[1][2][3]

 

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase to induce partial spheroplast formation.[4] Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest the cell walls of yeast and generate spheroplasts from fungi for transformation. It contains β-(1→3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities.[5] Lyticase is used for yeast cells like Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.[6]

溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。

 

一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA₈₀₀ 。

 

部分纯化的冻干粉末，含有磷酸钾缓冲盐和稳定剂

 

更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer